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Series GSE108786 Query DataSets for GSE108786
Status Public on Mar 26, 2018
Title ATAC-Seq analysis in vasopressin-responsive mouse renal collecting duct mpkCCD cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. Vasopressin binds a G protein-coupled receptor (V2R) activating several downstream signaling pathways. At downstream of V2R activation, many of transcription factors involve gene transcription process associated with status of chromatin structure. ATAC-Seq (Assay for Transpoase-Accessible Chromatin using Sequencing) is a recent technique to study chromatin accessibility (Buenrostro et al. Nat Methods 2013). We carried out ATAC-Seq following standard an ATAC-Seq protocol in mpkCCD cells treated with vehicle or dDAVP for 30 minutes.
Overall design To identify Tn5-accessible chromatin regions where transcription factors may bind, we carried out ATAC-seq . Observations were made both after 30 minutes treatment with the vasopressin analog dDAVP or vehicle (n=2 for each group).
Contributor(s) Jung H, Knepper MA
Citation(s) 29572403
Submission date Jan 04, 2018
Last update date Feb 11, 2019
Contact name Hyun Jun Jung
Organization name Johns Hopkins University School of Medicine
Department Medicine (Nephrology)
Street address 720 Rutland Ave, Ross Research Building 1165B
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM2913206 Rep1_vehicle_ATACSeq
GSM2913207 Rep1_dDAVP_ATACSeq
GSM2913208 Rep2_vehicle_ATACSeq
BioProject PRJNA428530
SRA SRP130938

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Supplementary file Size Download File type/resource
GSE108786_RAW.tar 8.3 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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