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Series GSE108798 Query DataSets for GSE108798
Status Public on Aug 31, 2018
Title Nuclear miRNA-mediated gene silencing dependent on Argonaute proteins is required for embryonic stem cell differentiation [4_Cnot shRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here, we show that embryonic stem cells (ESCs) and muscle stem cells contain high levels of nuclear AGO proteins and that in ESCs nuclear AGO2 protein activity is sufficient to allow for differentiation. Transcriptome-wide mapping of binding sites at nucleotide resolution revealed that nuclear AGO2 expands its target sequence repertoire in a miRNA-dependent manner to include intronic sequences to induce post-transcriptional gene silencing
 
Overall design shRNA CNOT1 and CNOT7 and RNA-seq from nuclear fraction
 
Contributor(s) Sarshad AA, Juan AH, Correa Muler AI, Anastasakis D, Wang X, Genzor P, Feng X, Tsai P, Sun H, Haase AD, Sartorelli V, Hafner M
Citation(s) 30146314
Submission date Jan 05, 2018
Last update date Feb 11, 2019
Contact name Markus Hafner
E-mail(s) Markus.hafner@nih.gov
Phone 3014026956
Organization name NIH
Department NIAMS
Lab Markus Hafner
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM2913346 ESC_NoTarget_shRNA rep1 (4)
GSM2913347 ESC_NoTarget_shRNA rep2 (4)
GSM2913348 ESC_Cnot1_shRNA rep1 (4)
This SubSeries is part of SuperSeries:
GSE108801 Nuclear miRNA-mediated gene silencing dependent on Argonaute proteins is required for embryonic stem cell differentiation
Relations
BioProject PRJNA428613
SRA SRP128098

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108798_CNOT1_CNOT7_shRNA.xlsx 4.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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