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Series GSE109613 Query DataSets for GSE109613
Status Public on Jan 25, 2018
Title Transcriptome analysis of isoxaben-induced loss of cell wall integrity in Arabidopsis nia1/2 mutant
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plant cell walls maintain their functional integrity during growth, defense and turgor generation through a dedicated cell wall integrity (CWI) maintenance mechanism. Upon detection of integrity impairment due to cell wall damage (CWD) compensatory responses are activated to maintain CWI. Responses include production of salicylic acid (SA), jasmonic acid (JA) and lignin as well as growth inhibition. Here we investigated the processes responsible for inducing growth inhibition upon CWD in Arabidopsis thaliana seedlings. Genetic studies implicated NITRATE REDUCTASE1 2 (NIA1 2) in the response to CWD. RNAseq-based transcriptomics showed that nearly all the CWD-induced transcript changes detectable in Col-0 were absent in nia1 2 seedlings.
Overall design Both genotypes (Col-0 and nia1/2) were treated with either isoxaben or DMSO (mock). Three replicates of Col-0 samples and two replicates of nia1/2 samples were analyzed.
Contributor(s) Gigli-Bisceglia N, Engelsdorf T, Vaahtera L, Hamann T
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Submission date Jan 24, 2018
Last update date Sep 20, 2018
Contact name Thorsten Hamann
Organization name NTNU
Department Department of Biology
Street address Høgskoleringen 5
City Trondheim
ZIP/Postal code 7491
Country Norway
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (10)
GSM2947236 Col-0 DMSO_rep1
GSM2947237 Col-0 DMSO_rep2
GSM2947238 Col-0 DMSO_rep3
BioProject PRJNA431488
SRA SRP131256

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Supplementary file Size Download File type/resource
GSE109613_RAW.tar 13.8 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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