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Series GSE10962 Query DataSets for GSE10962
Status Public on Apr 03, 2008
Title Herpes simplex host shutoff attenuates antiviral state
Platform organism Human alphaherpesvirus 1
Sample organism Mus musculus
Experiment type Expression profiling by array
Summary Herpes simplex virus mutants lacking the vhs gene are severely attenuated in animal models of pathogenesis and exhibit reduced growth in primary cell culture. As a result of these properties vhs-deleted virus have been proposed as live-attenuated viruses. Despite these findings and their implications for vacccines, the mechanisms by which vhs promotes infection in cell culture and in vivo are not understood. In this study we demonstrate that vhs-deficent viruses replicate to reduced levels in interferon(IFN)- primed cells. Furthermore, vhs-defective viruses induce increased levels of IFNβ and IFNβ-stimulated genes, and increased levels of eIF2α phosphorylation in infected cells. In addition, we demonstrate a generalized over-expression of viral RNAs following infection with a vhs-deficient virus. This suggests increased expression of IFN pathway inducing double stranded RNA, a potent pathogen-associated molecular pattern (PAMP). Together these data show that vhs likely functions to reduce innate immune responses and thereby acts as critical determinant of viral pathogenesis.
Keywords: time course, genetic modification
Overall design Time course (1,3,6,9 & 12h) of HSV infected mouse embryo fibroblasts. Wild type (KOS) virus is co-hybridized with vhs null virus (NHB). Each time-point is hybridized in quadruplicate.
Contributor(s) Crosby SD, Pasieka T, Alexander DE, Leib DA
Citation(s) 18367525
Submission date Mar 27, 2008
Last update date Mar 19, 2012
Contact name Seth Daniel Crosby
Phone 314-286-1256
Organization name Washington Univeristy School of Medicine
Department Genetics
Street address 660 S. Euclid
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL6599 WUSTL Human Herpesvirus 1
Samples (20)
GSM277653 1hour-A
GSM277654 1hour-A ds
GSM277655 3hour-A
BioProject PRJNA107163

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Supplementary file Size Download File type/resource
GSE10962_RAW.tar 2.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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