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| Status |
Public on Jul 01, 2019 |
| Title |
Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Maternal Vitamin C is required in vivo for proper DNA demethylation and development of fetal germ cells in a mouse model of Vitamin C deficiency. Withdrawal of Vitamin C from the maternal diet does not affect overall embryonic development but leads to defects in the fetal germline, which persist well after Vitamin C re-supply during late gestation. The transcriptome of germ cells from Vitamin C-deficient embryos is remarkably similar to that of embryos carrying a mutation in Tet1, which is responsible for DNA demethylation and activation of regulators of meiosis. In agreement with these results, Vitamin C deficiency leads to an aberrant DNA methylation profile that includes incomplete demethylation of key regulators of meiosis and transposable elements. These findings reveal that deficiency in Vitamin C during gestation recapitulates a mutation in Tet1 and disrupts germline reprogramming and development. Our work further indicate that the embryonic germline is sensitive to perturbations of the maternal diet, providing a potential intergenerational mechanism for adjusting fecundity to environmental quality.
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| |
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| Overall design |
Examination of the DNA methylation (RRBS) profiles of single E13.5 female Gulo -/- embryos developed with and without Vitamin C supplementation. 6 biological replicates per condition.
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| |
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| Contributor(s) |
Parker SL, Ramalho-Santos M |
| Citation(s) |
31485074 |
| |
| Submission date |
Jan 28, 2018 |
| Last update date |
Sep 19, 2019 |
| Contact name |
Stephanie L Parker |
| E-mail(s) |
slparker87@gmail.com
|
| Organization name |
UCSF
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE109747 |
Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline |
|
| Relations |
| BioProject |
PRJNA431944 |
| SRA |
SRP131697 |
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