GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE109874 Query DataSets for GSE109874
Status Public on Jun 07, 2019
Title Histone methyltransferase PRDM9 is not essential for meiosis in male mice
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A hallmark of meiosis is the rearrangement of parental alleles to assure genetic diversity in gametes. These chromosome rearrangements are mediated by the repair of programmed DNA double-strand-breaks (DSBs) as genetic crossovers between parental homologs. In mice, humans, and many other mammals, meiotic DSB occur primarily at hotspots, determined by sequence-specific binding of the PRDM9 protein. Without PRDM9, meiotic DSBs occur near gene promoters and other functional sites. Studies in a limited number of mouse strains showed that functional PRDM9 is required to complete meiosis, but despite its apparent importance, Prdm9 has been repeatedly lost across many animal lineages. Both the reason for mouse sterility in the absence of PRDM9 and the mechanism by which Prdm9 can be lost remain unclear. Here, we explore if mice can tolerate the loss of Prdm9. By generating Prdm9 functional knockouts in an array of genetic backgrounds, we observe a wide range of fertility phenotypes and ultimately demonstrate that PRDM9 is not required for completion of meiosis. Although DSBs still form at a common subset of functional sites in all mice lacking PRDM9, meiotic outcomes differ substantially. We speculate that DSBs at functional sites are difficult to repair as a crossover and that by increasing the efficiency of crossover formation at these sites, genetic modifiers of recombination rates can allow for meiotic progression. This model implies that species with a sufficiently high recombination rate may lose Prdm9 yet remain fertile.
Overall design DSB hotspots were mapped in two replicates each of B6 Prdm9-/-, PWD Prdm9-/- and (B6xPWD)F7 Prdm9-/- mice.
Contributor(s) Mihola O, Pratto F, Brick K, Linhartova E, Kobets T, Flachs P, L.Baker C, Sedlacek R, Paigen K, Petkov PM, Camerini-Otero RD, Trachtulec Z
Citation(s) 31186301
Submission date Jan 30, 2018
Last update date Sep 06, 2019
Contact name Kevin Brick
Organization name NIDDK
Department GBB
Street address 5/205 Memorial Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM2971933 B6 Prdm9ko DMC1 SSDS (A)
GSM2971934 B6 Prdm9ko DMC1 SSDS (B)
GSM2971935 B6xPWD F7 Prdm9ko DMC1 SSDS (A)
BioProject PRJNA432219
SRA SRP131785

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109874_RAW.tar 5.3 Gb (http)(custom) TAR (of BAI, BAM, BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap