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Series GSE109897 Query DataSets for GSE109897
Status Public on Jun 29, 2018
Title Facultative dosage compensation of developmental genes on autosomes in Drosophila and mammals [ChIP-seq mouse]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Haploinsufficiency and aneuploidy are two phenomena, where alteration of gene dosage causes severe cellular defects ultimately resulting in developmental failures and disease. One remarkable exception is the X chromosome, where copy number differences between males and females are buffered through the action of dosage compensation systems. In Drosophila, the Male-Specific Lethal complex (MSLc) mediates two-fold upregulation of the single male X chromosome via Histone H4 lysine 16 acetylation (H4K16ac). The evolutionary origin and conservation of this process orchestrated by MSL2, the only male-specific protein within the fly MSLc, have remained unclear. Here, we report that MSL2, in addition to its function on the X, targets dosage-sensitive autosomal genes involved in patterning and morphogenesis. We show that the precise regulation of these genes by MSL2 is required for proper development of the fly wing. This set of dosage sensitive genes maintained such regulation during evolution, as MSL2 binds and similarly regulates mouse orthologues via deposition of H4K16ac. We propose that MSL2-mediated H4K16ac is an evolutionarily conserved process mediating gene-by-gene dosage compensation across flies and mammals.
 
Overall design RNA sequencing of mouse embryonic stem cells of control and two indiviual msl2 knock out cell lines
 
Contributor(s) Valsecchi CI, Semplicio G, Akhtar A
Citation(s) 31601616
Submission date Jan 30, 2018
Last update date Nov 25, 2019
Contact name Asifa Akhtar
E-mail(s) akhtarlab_data@ie-freiburg.mpg.de
Organization name Max Planck Institute of Immunobiology and Epigenetics
Department Chromatin Regulation
Lab Akhtar Lab
Street address Stuebeweg 51
City Freiburg
ZIP/Postal code 79108
Country Germany
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (4)
GSM2973438 mESC_Msl2KO_H3_ChIP
GSM2973439 mESC_Msl2KO_H4K16ac_ChIP
GSM2973440 mESC_WT_H3_ChIP
This SubSeries is part of SuperSeries:
GSE109901 Facultative dosage compensation of developmental genes on autosomes in Drosophila and mammals
Relations
BioProject PRJNA432258
SRA SRP131810

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Supplementary file Size Download File type/resource
GSE109897_mESC_Msl2KO_K16ac_vs_H3_log2FC.bw 2.2 Gb (ftp)(http) BW
GSE109897_mESC_WT_K16ac_vs_H3_log2FC.bw 2.6 Gb (ftp)(http) BW
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Raw data are available in SRA
Processed data are available on Series record

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