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Series GSE110065 Query DataSets for GSE110065
Status Public on Feb 03, 2018
Title Comparison of primary vs immortalized murine hepatocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors and species. Cell type specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research and tailored drug development.
In these experiments primary murine hepatocytes were compared to immortalized murine hepatocytes with respect to their global gene expression pattern.
Overall design The global gene expression profile of primary and immortalized hepatocytes (cell line e-mHepA) was analyzed. In addition the murine hepatocyte cell line Hepa1-6 and murine fibroblasts were included as controls (two samples each). The immortalized murine hepatocytes were cultivated on normal tissue cultures plastic (2D samples) and as spheroids (3D samples). The hepatocyte cell line e-mHepA was immortalized by the genes TAg, KLF4, ID3 and Core. Each sample was run in duplicate.
Contributor(s) Lipps C, Wahlicht T, Dittrich-Breiholz O, May T, Wirth D
Citation(s) 29520052
Submission date Feb 02, 2018
Last update date May 05, 2018
Contact name Oliver Dittrich-Breiholz
Organization name Medical School Hannover
Department Research Core Unit Genomics
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (10)
GSM2977241 e-mHepA2D_1
GSM2977242 e-mHepA2D_2
GSM2977243 e-mHepA3D_1
BioProject PRJNA432701

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE110065_RAW.tar 21.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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