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| Status |
Public on Feb 10, 2018 |
| Title |
Effect of EcR RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We compared ecdysone receptor (EcR)-dependent gene expression in the Drosophila fat body on 0.15 M sucrose and 0.5 M sucrose high-sugar diets in order to gain insight into the role of this gene during caloric overload. Phenotypic analyses showed an increased severity of EcR RNAi phenotypes with increasing dietary sugar concentration. Because EcR is a transcription factor, we performed RNA-seq studies to identify transcriptional targets that might underlie insulin resistance downstream of EcR RNAi.
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| Overall design |
Control and fat body-expressed EcR RNAi Drosophila were reared on control (0.15 M sucrose) and high-sugar (0.5 M sucrose) diets until the wandering stage. Fat bodies were isolated and RNA extracted to determine the effects of diet on gene expression using Illumina RNA-seq.
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| Contributor(s) |
Musselman L, Baranski T |
| Citation(s) |
29487137 |
| |
| Submission date |
Feb 09, 2018 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Laura Palanker Musselman |
| E-mail(s) |
palankl@hotmail.com
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| Organization name |
Binghamton University
|
| Department |
Biological Sciences
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| Lab |
S3 room 210
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| Street address |
4400 Vestal Parkway East
|
| City |
Binghamton |
| State/province |
New York |
| ZIP/Postal code |
13902 |
| Country |
USA |
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| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA433687 |
| SRA |
SRP132604 |
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