|
| Status |
Public on Feb 15, 2018 |
| Title |
Identification of the tumour transition states occurring during EMT [p63 overexpression RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
EMT in cancer has been associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However,direct in vivo evidence supporting this possibility is still lacking. By screening a large panel of cell surface markers, we identified the existence of multiple tumour subpopulations associated with different EMT stages from epithelial to completely mesenchymal states passing through intermediate hybrid states in skin and mammary primary tumours. Although all EMT subpopulations presented similar tumour propagating cell capacity, they displayed different , invasiveness and metastatic potential. Their transcriptional and epigenetic landscapes defined by RNA-seq and ATAC-seq identified the underlying gene regulatory networks, transcription factors and signalling pathways that control these different EMT transition states. Finally, these tumour subpopulations are localized in different niches that differentially regulateEMT transition states.
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| |
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| Overall design |
RNA sequencing on the different intermediate states of EMT after overexpression of p63
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| |
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| Contributor(s) |
Pastushenko I, Brisebarre A, Sotiropoulou P, Blanpain C |
| Citation(s) |
29670281 |
| |
| Submission date |
Feb 14, 2018 |
| Last update date |
Sep 12, 2018 |
| Contact name |
Cédric Blanpain |
| E-mail(s) |
cedric.blanpain@ulb.ac.be
|
| Organization name |
ULB
|
| Lab |
Laboratory of Stem Cells and Cancer
|
| Street address |
Route de Lennik 808
|
| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE110587 |
Identification of the tumour transition states occurring during EMT |
|
| Relations |
| BioProject |
PRJNA434065 |
| SRA |
SRP132845 |
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