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Series GSE110860 Query DataSets for GSE110860
Status Public on Mar 19, 2019
Title Chromatin accessibility in C. albicans stimulated HoxER-PU.1 WT and HoxER-PU.1 KO neutrophils and DMSO, TSA or Entinostat treated HoxER-PU.1 WT neutrophils
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Neutrophils are essential first line defense cells against invading pathogens, yet their inappropriate activation contributes to immunological diseases and can cause collateral tissue damage. However, if and how neutrophils cell-intrinsically titrate their inflammatory response remains unknown. Here, we conditionally deleted PU.1, a key myeloid transcription factor, from the neutrophils of mice undergoing fungal infection, and then performed comprehensive epigenomic profiling. We find that a major function of PU.1 is to restrain the neutrophils’ immune response by broadly suppressing genomic enhancer outputs via recruiting histone deacetylase activity, thereby limiting the immune-stimulatory AP1-transcription factor JUNB from entering chromatin. Thus, neutrophils rely on a direct PU.1 repressor function as rheostat of the inflammatory chromatin state, safeguarding their epigenome from undergoing uncontrolled activation prior to pathogenic stimulation.
Overall design Tn-Seq profiles of 2 hours C. albicans stimulated wildtype and PU.1 deleted neutrophils as well as DMSO, TSA or Entinostat treated wildtype neutrophils were generated by deep sequencing in duplicates.
Contributor(s) Rosenbauer F, Fischer J, Walter C, Tönges A
Citation(s) 30911105
Submission date Feb 20, 2018
Last update date Oct 28, 2021
Contact name Frank Rosenbauer
Organization name University Hospital Münster
Department Institute of Molecular Tumor Biology
Street address Robert-Koch-Str. 43
City Münster
ZIP/Postal code 48149
Country Germany
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (13)
GSM3017953 ATACseq_unstimulated_WT_rep1
GSM3017954 ATACseq_unstimulated_WT_rep2
GSM3017955 ATACseq_unstimulated_KO_rep1
This SubSeries is part of SuperSeries:
GSE110865 Safeguard function of PU.1 shapes the inflammatory epigenome of neutrophils
BioProject PRJNA434709
SRA SRP133138

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Supplementary file Size Download File type/resource
GSE110860_ATAC_ffU_peaks.bed.gz 482.5 Kb (ftp)(http) BED
GSE110860_ATAC_wtU-wtCa_wtCa_spec_peaks.bed.gz 15.2 Kb (ftp)(http) BED
GSE110860_ATAC_wtU-wtCa_wtU_spec_peaks.bed.gz 101 b (ftp)(http) BED
GSE110860_ATAC_wtU_peaks.bed.gz 298.1 Kb (ftp)(http) BED
GSE110860_DMSO_rep1_rmdup_cutSites.wig.gz 199.4 Mb (ftp)(http) WIG
GSE110860_DMSO_rep2_rmdup_cutSites.wig.gz 216.8 Mb (ftp)(http) WIG
GSE110860_Enti_rep1_rmdup_cutSites.wig.gz 191.3 Mb (ftp)(http) WIG
GSE110860_Enti_rep2_rmdup_cutSites.wig.gz 171.4 Mb (ftp)(http) WIG
GSE110860_TSA_rmdup_cutSites.wig.gz 161.5 Mb (ftp)(http) WIG
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