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Series GSE110884 Query DataSets for GSE110884
Status Public on Feb 21, 2018
Title The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Oncogenic signals can induce premature senescence (OIS) in normal human cells causing a proliferation arrest and the elimination of these defective cells by immune cells. In order to identify new regulators of OIS, we performed a loss-of-function genetic screen and identified that the loss of SCN9A sodium channel allowed cells to escape from OIS. Here we studied the transcriptome profiles of an OIS model based on human mammary epithelial cells stably expressing hTert to be immortalized and MEK:ER, a 4-hydroxytamoxifen (4-OHT) inducible oncogene MEK:ER (HEC-TM cells), to induce the oncogenic signal. We used an shRNA in order to knockdown SCN9A expression during OIS, and KCL treatment to study the impact of membrane depolarization on senescence induction. We showed that SCN9A and plama membrane depolarization mediated the repression of mitotic genes through a calcium/Rb/E2F pathway to promote senescence. Taken together, our work delineates a new pathway, which involves the NFkB transcription factor, SCN9A expression, plasma membrane depolarization.
Overall design For each condition three independent biological replicates were analysed. Untreated HEC-TM cells condition have been used as control and the data have been normalized on this control.
Contributor(s) Flaman J, Bernard D
Citation(s) 29446526
Submission date Feb 20, 2018
Last update date May 23, 2018
Contact name jean-michel flaman
Phone +33687477812
Organization name Inserm 1052
Department CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69373
Country France
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (15)
GSM3018250 HEC-TM_R1
GSM3018251 HEC-TM_R2
GSM3018252 HEC-TM_R3
BioProject PRJNA434736

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Supplementary file Size Download File type/resource
GSE110884_RAW.tar 32.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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