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| Status |
Public on Feb 20, 2020 |
| Title |
Expression analysis of MADM based glioma mouse model and normal brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mosaic Analysis with Double Markers (MADM) based glioma mouse model, which homozygously lacks Tp53 and Nf1, spontaneously developed gliomas at the post-natal 90-120 days. Tp53 and Nf1 are among the most frequently mutated genes in human glioma patients. Investigating the expression changes of genes induced by inactivation of Tp53, Nf1 and EZH2 can be a clue to clarify the mechanism of gliomagenesis. We examined the expressions of glioma cells in MADM mouse (n=2), EZH2 knocked-out MADM glioma tissues (n=3) and neural stem cell (NSC), astrocyte established with normal mice brains (n=2, respectively) and TP53 knocked-out astrocytes, derived from TP53 knocked-out mice (n=2). We used SurePrint G3 Mouse GE 8×60K array slides (G4858A, Agilent Technologies).
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| Overall design |
Microarray experiment was performed as single-color hybridization on SurePrint G3 Mouse GE 8 x 60K array.
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| Contributor(s) |
Ohka F, Deguchi S, Shinjo K, Katsushima K, Wakabayashi T, Natsume A, Kondo Y |
| Citation(s) |
31431463 |
| |
| Submission date |
Feb 21, 2018 |
| Last update date |
May 21, 2020 |
| Contact name |
Keisuke Katsushima |
| E-mail(s) |
kkeisuke@med.nagoya-cu.ac.jp
|
| Phone |
+81-052-853-8196
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| Organization name |
Nagoya City University Graduate School of Medical Sciences
|
| Department |
Department of Epigenomics
|
| Lab |
Yutaka Kondo
|
| Street address |
1 Kawasumi, Mizuho-cho, Mizuho-ku
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| City |
Nagoya |
| ZIP/Postal code |
467-8601 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE110937 |
ChIP and expression analysis of MADM based glioma mouse model |
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| Relations |
| BioProject |
PRJNA434828 |
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