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Series GSE111127 Query DataSets for GSE111127
Status Public on Feb 26, 2019
Title The downstream gene expression of Notch signal in tumor associated endothelial cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To study the effects of Notch signal in tumor associated endothelial cells ,we conducted a RNA sequence assay. Briefly, TECs were isolatedas described above from LLC tumors 14 days after inoculation in 3 pairs of NICeCA and control mice. Total RNA was extracted using Trizol, and RNA integrity was evaluated using the Agilent 2200 Tape Station (Agilent Technologies, Santa Clara, California, USA) and each sample had the RINe above 7.0. rRNA was removed using the EpicentreRibo-Zero rRNARemoval Kit ( Illumina, San Diego, CA). Remaining RNA was fragmented into approximately 200bp fragments. Subsequently, the sample was subjected to first and second strand cDNA synthesis followed by adaptor ligation and enrichment with a low-cycle PCR usingTruSeq® RNA LT/HT Sample Prep Kit (Illumina). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0 (Life Technologies) and then diluted to 10 pM for cluster generation in situ on the HiSeq3000 pair-end flow cell followed by sequencing (2×150 bp) on HiSeq 3000.
Overall design LLC tumor endothelial cells mRNA profile of endothelial specific Notch signal activation mice and control mice were generated by deep sequencing, in triplicate,using Illumina 3000
Contributor(s) Yan X, Han H
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Submission date Feb 26, 2018
Last update date Feb 26, 2019
Contact name Xian-Chun Yan
Phone +86-15891702964
Organization name fourth military medical university
Street address Chang-Le Xi street #169
City Xi'an
ZIP/Postal code 710032
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM3022573 Ctrl rep1
GSM3022574 Ctrl rep2
GSM3022575 Ctrl rep3
BioProject PRJNA435984
SRA SRP133491

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Supplementary file Size Download File type/resource
GSE111127_gene-exp.txt.gz 2.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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