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Series GSE111667 Query DataSets for GSE111667
Status Public on Mar 13, 2018
Title miRNA expression in lung tissue
Organism Mus musculus
Experiment type Other
Summary Sex differences in the incidence and prognosis of respiratory diseases have been reported. Studies have shown that women are at increased risk of adverse health outcomes from air pollution than men, but sex-specific immune gene expression patterns and regulatory networks have not been well studied in the lung. MicroRNAs (miRNAs) are environmentally sensitive posttranscriptional regulators of gene expression that may mediate the damaging effects of inhaled pollutants in the lung, by altering the expression of innate immunity molecules. Here, male and female mice of the C57/Bl6 background were exposed to 2ppm of ozone or filtered air (control) for 3 hours. Female mice were also exposed at different stages of the estrous cycle. Following exposure, lungs were harvested and total RNA was extracted. We used PCR arrays to study sex-differences in the expression of 84 miRNAs predicted to target inflammatory and immune genes. Our results identified differentially expressed miRNA signatures in the lungs of male vs. female exposed to ozone. In silico pathway analyses identified sex-specific biological networks affected by exposure to ozone that ranged from direct predicted gene targeting to complex interactions with multiple intermediates. We also identified differences in miRNA expression and predicted regulatory networks in females exposed to ozone at different estrous cycle stages. These results indicate that both sex and hormonal status can influence lung miRNA expression in response to ozone exposure, indicating that sex-specific miRNA regulation of inflammatory gene expression could mediate differential pollution-induced health outcomes in men and women.
Overall design Male and female C57BL/6 mice of 8 weeks of age were exposed to 2ppm ozone (O3) or filtered air (FA) for 3 hours. Females were exposed at different stages of the estrous cycle: diestrus 1 (D1), diestrus 2 (D2), proestrus (P), estrus (E). Total RNA was extracted from whole lung homogenates and PCR arrays were used to determine the expression of 84 miRNAs associated with inflammation and immunity (3-9 animals per condition).
Contributor(s) Fuentes N, Silveyra P
Citation(s) 29739446
NIH grant(s)
Grant ID Grant title Affiliation Name
K01 HL133520 Sex-specific mechanisms of ozone-induced acute lung inflammation PENNSYLVANIA STATE UNIV HERSHEY MED CTR Patricia Silveyra
K12 HD055882 Career Development Program in Women's Health Research at Penn State PENNSYLVANIA STATE UNIV HERSHEY MED CTR CAROL S WEISMAN
Submission date Mar 12, 2018
Last update date May 10, 2018
Contact name Patricia Silveyra
Organization name Indiana University Bloomington
Department Environmental and Occupational Health
Lab Silveyra lab
Street address 2819 E 10th St
City Bloomington
State/province Indiana
ZIP/Postal code 47408
Country USA
Platforms (1)
GPL24720 Qiagen Mouse Inflammatory Response & Autoimmunity miRNA PCR Array (MIMM-105Z)
Samples (56)
GSM3036830 SAMPLE 7: male_FA_3h_rep1
GSM3036831 SAMPLE 8: male_FA_3h_rep2
GSM3036832 SAMPLE 9: male_O3_3h_rep1
BioProject PRJNA437828

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Supplementary file Size Download File type/resource
GSE111667_results_tables.xls.gz 134.8 Kb (ftp)(http) XLS
Processed data included within Sample table
Processed data are available on Series record

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