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Series GSE111716 Query DataSets for GSE111716
Status Public on Feb 12, 2019
Title TMK1-mediated transcriptional auxin signaling guides differential growth in plant
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Auxin plays diverse and profound roles in plants, regulating essentially all aspects of plant growth and development. Yet how auxin achieves such functional diversity and specificity is largely unknown. Here, we address how different concentrations of auxin determine developmental responses through auxin signaling pathways, which are essential for differential growth including apical hook development. We showed that a high auxin concentration at concave side of the apical hook stimulated the C-terminus cleavage and cytosolic translocation of Transmembrane Kinase 1 (TMK1), which then phosphorylates non-canonical IAA32 and IAA34. Surprisingly, and in contrast to the previous report that auxin degrades IAA proteins through the TIR1/AFB auxin receptor to regulate gene expression, here we found that non-canonical IAA32 and IAA34 proteins were stabilized by the high auxin concentration through TMK1 cleavage to negatively regulate gene expression and inhibit growth. Thus, we demonstrated that TIR1/AFB-independent transcriptional auxin signaling regulated growth inhibition in response to the high auxin concentration.
Overall design mRNA profiles of Col-0, tmk1-1 and iaa32iaa34 apical hook region
Contributor(s) Cao M
Citation(s) 30944466
Submission date Mar 12, 2018
Last update date May 26, 2019
Contact name Renyi Liu
Phone 02157078228
Organization name Shanghai Center for Plant Stress Biology
Department Bioinformatics
Street address 3888 Chenhua Road
City Shanghai
State/province Shanghai
ZIP/Postal code 201602
Country China
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (9)
GSM3037960 WT-1
GSM3037961 WT-2
GSM3037962 WT-3
BioProject PRJNA437964
SRA SRP134963

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Supplementary file Size Download File type/resource
GSE111716_Table_CPM_9Ath.txt.gz 763.3 Kb (ftp)(http) TXT
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