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Series GSE111741 Query DataSets for GSE111741
Status Public on Mar 13, 2018
Title RNA-seq analysis of colorectal cancer cell line (CRC) SW1116 overexpressed POLR1D
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Gene expression profiles were generated for CRC cell line SW1116 overexpressed POLR1D and empty vector to explore the effect of POLR1D on colorectal cancer cell line SW1116.
Overall design SW1116 cells were infected with lentivirus overexpressing POLR1D and negative control lentivirus. After 72h, the GFP-positive cells were isolated by FACS. POLR1D overexpression was confirmed by RT-PCR and Western blotting. Then, the total RNA of POLR1D overexpression cells and negative control cells was extracted by Trizol reagent and sequenced using Illumina HiSeqTM4000 according to the manufacturer’s instructions.
Contributor(s) Wang M, Niu W
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Submission date Mar 12, 2018
Last update date Mar 21, 2019
Contact name wenbo niu
Organization name southern medical university
Street address shatai road 1023
City guangzhou
ZIP/Postal code 510515
Country China
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (2)
GSM3038962 Control group
GSM3038963 POLR1D overexpression group
BioProject PRJNA438017
SRA SRP135144

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111741_RAW.tar 430.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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