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| Status |
Public on Feb 06, 2019 |
| Title |
Genome-wide RUNX1 binding landscape in AMLs with RUNX1 mutation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Analyses of 38 AML samples through integrated multiple epigenomic analysis exposes two major epigenetic subtypes. We found that the majority of patients in an AML subtype have molecular aberrations associated with RUNX1 and splicing factors. Despite this heterogeneity, they give rise to a comparable epigenome, suggesting a common deregulation of the epigenome. Given that differentially spliced genes could result in truncated proteins and/or reduced protein levels, we speculated that mutated RUNX1 protein might deregulate the same genes targeted by mutated spliceosome factors. To explore this option, we performed genome-wide binding analysis of RUNX1 in the RUNX1 mutant (RUNX1mt) expressing AMLs, and analyzed its relationship with previous epigenetic results in our study.
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| |
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| Overall design |
Four AML samples carrying RUNX1 mutation were sequenced
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| |
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| Contributor(s) |
Martens JH |
| Citation(s) |
30709863 |
| |
| Submission date |
Mar 14, 2018 |
| Last update date |
Nov 11, 2021 |
| Contact name |
Joost Martens |
| E-mail(s) |
j.martens@science.ru.nl
|
| Phone |
0243780645
|
| Organization name |
Radboud University
|
| Department |
RIMLS
|
| Lab |
Molecular Biology
|
| Street address |
Geert Grooteplein 28
|
| City |
Nijmegen |
| State/province |
Nederland |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA438279 |
| SRA |
SRP135672 |
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