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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 01, 2018 |
| Title |
Genomic integrity safeguards pluripotency in embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Illumina BeadChip array analyses of mouse embryonic stem cell gene expression profiles in the presence of or upon knockdown of 4 candidate factors regulating genomic integrity -- Chek1, Ppm1g, Ppp2r1b and Bub1b. An ES complementation 'rescue' system was employed to measure the effects of knockdown (minus Dox). The 4 candidate factors are rescued to wildtype levels in the presence of Dox (plus Dox). A control rescue ES cell line, with a Luciferase-targeting shRNA, was also assessed.
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| Overall design |
Total of 30 samples; Chek1_R (+/-Dox), Ppm1g_R (+/-Dox), Ppp2r1b_R (+/-Dox), Bub1b_R (+/-Dox), Control_R (+/-Dox); all performed in triplicates.
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| Contributor(s) |
Lee DF, Su J, Ang YS, Carvajal-Vergara X, Chang B, Sevilla A, Zhao R, Schaniel C, Lemischka IR |
| Citation(s) |
31390555 |
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| Submission date |
Mar 18, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Yen-Sin Ang |
| Organization name |
Gladstone Institute, UCSF
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| Street address |
1650 Owens Street
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| City |
san francisco |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA438830 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE111982_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE111982_non-normalized.txt.gz |
3.1 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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