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Series GSE111986 Query DataSets for GSE111986
Status Public on Mar 18, 2021
Title A novel conserved transcription factor CLR-4 regulates cellulase genes expression through controlling the intracellular cAMP level in ascomycete fungi
Organisms Neurospora crassa; Thermothelomyces thermophilus
Experiment type Expression profiling by high throughput sequencing
Summary Fungal degradation of lignocellulosic biomass requires various (hemi-)cellulases and plays key roles in biological carbon cycle. Although cellulases induction recently described in some saprobic filamentous fungi, regulation of cellulase transcription has not been studied thoroughly. Here, we identified and characterized the novel cellulase regulation factors clr-4 in Neurospora crassa and its ortholog Mtclr-4 in Myceliophthora thermophila. Deletion of clr-4 and Mtclr-4 displayed similarly defective phenotypes in cellulolytic enzymes production and activities. Transcriptomics analysis of Δclr-4/ΔMtclr-4 revealed down-regulation of not only encoding genes of (hemi-)cellulases and pivotal regulators (clr-1, clr-2 and xyr-1), but also the key genes of cAMP signaling pathway such as adenylate cyclase cr-1. Consistently, the significant decreased levels of intracellular cAMP were observed in Δclr-4/ΔMtclr-4 compared to wild-type during cellulose utilization. Electrophoretic mobility shift assays (EMSA) verified that CLR-4 could directly bind to the promoter regions of adenylyl cyclase (Nccr-1) and cellulose regulator clr-1, while MtCLR-4 bind to upstream regions of adenylyl cyclase Mtcr-1 and biomass deconstruction regulators Mtclr-2 and Mtxyr-1. Concluded, the novel cellulase expression regulators (CLR-4/MtCLR-4) findings here significantly enrich our understanding of the regulatory network of cellulose degradation and provide new targets for industrial fungi strain engineering for plant biomass deconstruction in biorefinery.
Overall design mRNA profiles of Neurospra crassa and Myceliophthora thermophila cells exposed to 4h Avicel, respectively.
Contributor(s) Liu Q, Li J, Tian C
Citation(s) 30474279
Submission date Mar 19, 2018
Last update date Sep 16, 2022
Contact name Jingen Li
Phone +86-02284861948
Organization name Tianjin institute of industrial biotechnology,Chinses Academy of Sciences
Street address Xiqi Dao 32, Tianjin Airport Economic Area
City Tianjin
ZIP/Postal code 300308
Country China
Platforms (2)
GPL16164 Illumina HiSeq 2000 (Neurospora crassa)
GPL24572 Illumina HiSeq 2000 (Thermothelomyces thermophila)
Samples (6)
GSM3048706 Mtclr-4_4h_1
GSM3048707 Mtclr-4_4h_2
GSM3048708 MtWT_4h_1
BioProject PRJNA438883
SRA SRP135970

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Supplementary file Size Download File type/resource
GSE111986_Readcount.xlsx 1.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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