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Series GSE112127 Query DataSets for GSE112127
Status Public on Mar 01, 2019
Title Genome-wide assay of BRG1 regulated genes in inflamed colon epithelial cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we focus on the function of BRG1 in colitis and colitis-associated-cancer, in the aim of finding the direct target genes of BRG1 which may response for the phenotype we observed in BRG1 conditional knock-out mice, ChIP-Seq assay is used. The cells used for ChIP-Seq is colon epithelial cells which isolated from DSS treated mice.
Overall design To mimic the status of BRG1IEC KO mice, wild-type mice were treated with 2% DSS for 3 days and IECs were isolated by EDTA isolation buffer, and each sample contains pooled IECs from 5 mice. Flash frozen cells were then sent to Active Motif, Inc. for chromatin extraction and followed by immunoprecipitation using anti-BRG1 antibody (Abcam, cat # ab110641).
Contributor(s) Liu Y, Qin J
Citation(s) 31601814
Submission date Mar 21, 2018
Last update date Oct 21, 2019
Contact name Yongfeng Liu
Organization name The Institute of Health Sciences, SIBS, CAS / SJTUSM
Street address 320 Yueyang Road, Shanghai, 200025 P.R. China
City shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM3058262 BRG1_ChIPseq
GSM3058263 colon epthelial cells input DNA
This SubSeries is part of SuperSeries:
GSE112128 Function of BRG1 in colitis and colitis-associated-cancer
BioProject PRJNA439398
SRA SRP136165

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112127_RAW.tar 283.7 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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