|
| Status |
Public on May 03, 2019 |
| Title |
RPL12/uL11 phosphorylation regulates translation during mitosis [RIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Emerging evidence indicates that heterogeneity in ribosome composition can give rise to specialized functions. Until now, research mainly focused on differences in core ribosomal proteins and associated factors. The impact of posttranslational modifications has not yet been studied systematically. Analyzing ribosome heterogeneity is challenging since individual proteins can be part of different subcomplexes (40S, 60S, 80S and polysomes). Here, we develop polysome proteome profiling to obtain unbiased proteomic maps across ribosomal subcomplexes. Our method combines extensive fractionation by sucrose gradient centrifugation with quantitative mass spectrometry. The high resolution of the profiles allows us to assign proteins to specific subcomplexes. Phosphoproteomics on the fractions reveals that phosphorylation of serine 38 in RPL12/uL11 -- a known mitotic CDK1 substrate -- is strongly depleted in polysomes. Follow-up experiments confirm that RPL12/uL11 phosphorylation regulates translation of specific subsets of mRNAs during mitosis. Together, our results show that posttranslational modification of ribosomal proteins can regulate translation.
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| |
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| Overall design |
Gene expression profiling of immunoprecipitated RPL12 protein-RNA complexes
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| |
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| Contributor(s) |
Imami K, Milek M, Bogdanow B, Kastelic N, Yasuda T, Landthaler M, Selbach M |
| Citation(s) |
30220558 |
| |
| Submission date |
Mar 21, 2018 |
| Last update date |
May 03, 2019 |
| Contact name |
Markus Landthaler |
| E-mail(s) |
markus.landthaler@mdc-berlin.de
|
| Organization name |
Max Delbrueck Center
|
| Department |
Berlin Institute for Medical Systems Biology
|
| Street address |
Hannoversche Str. 28
|
| City |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE112187 |
RPL12/uL11 phosphorylation regulates translation during mitosis |
|
| Relations |
| BioProject |
PRJNA445182 |
| SRA |
SRP136214 |
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