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| Status |
Public on Jan 07, 2019 |
| Title |
Subcellular localization analysis of long non-non coding RNAs (lncRNAs) in skeletal muscle myofibers |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
In order to study the subcellular localization of lncRNAs in myofibers of skeletal muscle, single myofibers were isolated from Soleus, Extensor Digitorum Longus (EDL) and Tibialis Anterior (TA) of 9 mice. RNA was extracted from nuclei and cytoplasmic fractions of 5-10 isolated myofibers and than separately hybridized on microarrays. Preferential expression in nuclear or cytoplasmic compartment of each lncRNA was determined.
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| Overall design |
Subcellular localization experiments were replicated using different pools of fibers from the same muscle of nine wild-type CD1 mice. Fibers were extracted from Soleus, EDL and TA for a total of 18 samples.
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| Contributor(s) |
Alessio E, Buson L, Chemello F, Grespi F, Martini P, Massimino ML, Pacchioni B, Millino C, Romualdi C, Bertoli A, Scorrano L, Lanfranchi G, Cagnin S |
| Citation(s) |
30649422 |
| |
| Submission date |
Apr 05, 2018 |
| Last update date |
Feb 04, 2019 |
| Contact name |
Gerolamo Lanfranchi |
| E-mail(s) |
stefano.cagnin@unipd.it
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| Phone |
+39-0498276219
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| Organization name |
University of Padova
|
| Department |
CRIBI - Biotechnology Center and Biology Department
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| Lab |
Functional Genomics Lab
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| Street address |
Via U. Bassi, 58/B
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL24842 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (re-annotated according to transcripts in Ensembl 74) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA448919 |
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