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Series GSE112769 Query DataSets for GSE112769
Status Public on Feb 21, 2019
Title Lymphoid Biased Hematopoietic Stem Cells are Maintained with Age and Retain Normal Developmental Potential in the Absence of Inflammatory Stimuli
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The hematopoietic stem cell (HSC) compartment includes lymphoid biased (Ly-HSCs) and myeloid biased (My-HSCs) subsets. Current models propose that the number of Ly-HSCs declines with age and this contributes to the diminution of lymphopoiesis that is a feature of aging. This view is based on a reduction in the frequency of Ly-HSCs in old bone marrow, but we now show that when total HSCs are taken into account, the number of Ly-HSCs is not reduced in old mice. We further demonstrate that old Ly-HSCs exhibit a normal capacity to produce lymphoid progenitors when removed from the bone marrow. However, the production of inflammatory cytokines is known to increase with age, and when Ly-HSCs are exposed to these mediators, lymphocyte production is blocked. We also demonstrate that old Ly-HSCs generate myeloid cells that phenotypically resemble those produced by old My-HSCs, a finding consistent with the myeloid biased gene expression pattern of old Ly-HSCs. These observations indicate that current models of how aging impacts the lymphopoietic potential of HSCs need to be revised and point to changes in the in vivo environment, rather than intrinsic changes in HSCs, as the principal cause of the reduced lymphopoiesis and increased myelopoiesis during aging.
Overall design We isolated Ly-HSCs (lineage negative, Sca-1+ CD117(c-kit)+ CD48- CD135- CD150low) and My-HSCs (lineage negative, Sca-1+ CD117(c-kit)+ (LSK) CD48- CD135- CD150high) from three independent groups of young (8-12 week-old) and old (18 months) mice and performed RNA-Seq.
Contributor(s) Kong Y, Montecino-Rodriguez E, Casero D, Dorshkind K, Pioli PD
Citation(s) 30799276
Submission date Apr 05, 2018
Last update date Mar 28, 2019
Contact name Kenneth Dorshkind
Phone 310 206-9535
Organization name David Geffen School of Medicine at UCLA
Department Pathology and Laboratory Medicine
Street address 10833 Le Conte Avenue
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM3083151 Old Lymphoid-biased HSCs #1
GSM3083152 Old Lymphoid-biased HSCs #2
GSM3083153 Old Lymphoid-biased HSCs #3
BioProject PRJNA448920
SRA SRP137765

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112769_Ly_My_HSCs.genesfpkm.txt.gz 772.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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