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Status |
Public on Mar 13, 2004 |
Title |
PDGF-induced genes regulated by the PI3K and MEK/ERK signaling pathways |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Human T98G glioblastoma cells were stimulated with platelet-derived growth factor (PDGF) and analyzed by DNA microarrays, which identified 74 immediate-early gene transcripts. Cells were then treated with inhibitors to identify subsets of genes that are targets of the phosphatidylinositol 3-kinase (PI3K) and MEK/ERK signaling pathways. Four groups of PDGF-induced genes were defined: independent of PI3K and MEK/ERK signaling, dependent on PI3K signaling, dependent on MEK/ERK signaling, and dependent on both pathways. Cells were grown in Minimal Essential Medium (Invitrogen) supplemented with fetal calf serum (10%). For growth factor/inhibitor treatments, cells were incubated in serum-free medium for 72 h, and either left unstimulated, or stimulated for 30 min with human PDGF-BB (50 ng/ml) (Sigma). U0126 (Cell Signaling Technology) and LY294002 (BioMol) were added 60 min prior to PDGF addition. Keywords: other
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Contributor(s) |
Tullai JW, Schaffer ME, Mullenbrock S, Kasif S, Cooper GM |
Citation(s) |
14769801 |
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Submission date |
Mar 11, 2004 |
Last update date |
Mar 02, 2012 |
Contact name |
Geoffrey M Cooper |
Organization name |
Boston University
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Department |
Biology
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Lab |
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Street address |
5 Cummington St
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
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Platforms (2) |
GPL875 |
Agilent Human 1 cDNA Microarray (G4100A) [layout A] |
GPL876 |
Agilent Human 1 cDNA Microarray (G4100A) [layout B] |
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Samples (11)
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GSM18502 |
72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 1 |
GSM18503 |
72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 2 |
GSM18504 |
72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 3 |
GSM18505 |
72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 4 |
GSM18506 |
72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 5 |
GSM18507 |
72 h serum-deprived T98G cells with 1 h LY294002 and 30 min PDGF treatments, replicate 1 |
GSM18508 |
72 h serum-deprived T98G cells with 1 h LY294002 and 30 min PDGF treatments, replicate 2 |
GSM18509 |
72 h serum-deprived T98G cells with 1 h U0126 and 30 min PDGF treatments, replicate 1 |
GSM18510 |
72 h serum-deprived T98G cells with 1 h U0126 and 30 min PDGF treatments, replicate 2 |
GSM18511 |
72 h serum-deprived T98G cells with 1 h LY294002 + U0126 and 30 min PDGF treatments, replicate 1 |
GSM18512 |
72 h serum-deprived T98G cells with 1 h LY294002 + U0126 and 30 min PDGF treatments, replicate 2 |
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Relations |
BioProject |
PRJNA87239 |
Supplementary file |
Size |
Download |
File type/resource |
GSE1128_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of TIFF) |
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