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Series GSE1128 Query DataSets for GSE1128
Status Public on Mar 13, 2004
Title PDGF-induced genes regulated by the PI3K and MEK/ERK signaling pathways
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human T98G glioblastoma cells were stimulated with platelet-derived growth factor (PDGF) and analyzed by DNA microarrays, which identified 74 immediate-early gene transcripts. Cells were then treated with inhibitors to identify subsets of genes that are targets of the phosphatidylinositol 3-kinase (PI3K) and MEK/ERK signaling pathways. Four groups of PDGF-induced genes were defined: independent of PI3K and MEK/ERK signaling, dependent on PI3K signaling, dependent on MEK/ERK signaling, and dependent on both pathways. Cells were grown in Minimal Essential Medium (Invitrogen) supplemented with fetal calf serum (10%). For growth factor/inhibitor treatments, cells were incubated in serum-free medium for 72 h, and either left unstimulated, or stimulated for 30 min with human PDGF-BB (50 ng/ml) (Sigma). U0126 (Cell Signaling Technology) and LY294002 (BioMol) were added 60 min prior to PDGF addition.
Keywords: other
 
 
Contributor(s) Tullai JW, Schaffer ME, Mullenbrock S, Kasif S, Cooper GM
Citation(s) 14769801
Submission date Mar 11, 2004
Last update date Mar 02, 2012
Contact name Geoffrey M Cooper
Organization name Boston University
Department Biology
Lab
Street address 5 Cummington St
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (2)
GPL875 Agilent Human 1 cDNA Microarray (G4100A) [layout A]
GPL876 Agilent Human 1 cDNA Microarray (G4100A) [layout B]
Samples (11)
GSM18502 72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 1
GSM18503 72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 2
GSM18504 72 h serum-deprived T98G cells with 30 min PDGF treatment, replicate 3
Relations
BioProject PRJNA87239

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Supplementary file Size Download File type/resource
GSE1128_RAW.tar 1.2 Gb (http)(custom) TAR (of TIFF)

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