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Series GSE112875 Query DataSets for GSE112875
Status Public on Apr 14, 2020
Title DNA 5-methylcytosine specific profiling with limited input
Organisms Homo sapiens; Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation 5mC specific detection has been limited by the mixed signals from traditional bisulfite sequencing or by the severe degradation of input during oxBS-seq pretreatment. Here, we presented a 5mC specific whole genome amplification method (5mC-WGA), with which we achieved whole genome bisulfite sequencing with 5mC retention from limited input down to 10 pg scale without 5hmC signals, presenting DNA 5mC methylome with high producibility and great accuracy.
Overall design We compared our 5mC-WGA method with well-established TAB-Seq and oxBS-Seq methods.
Contributor(s) Liu C, Cui X, Zhao B, He C
Citation(s) 32077696
Submission date Apr 09, 2018
Last update date Apr 14, 2020
Contact name Xiaolong Cui
Organization name Northwestern University
Department Department of Preventive Medicine
Street address 680 N Lake Shore Drive
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM4194635 WGABS_10pg_1
GSM4194636 WGABS_10pg_2
GSM4194637 WGABS_10pg_3
BioProject PRJNA449368

Download family Format
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Supplementary file Size Download File type/resource
GSE112875_RAW.tar 772.1 Mb (http)(custom) TAR (of COV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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