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Series GSE112939 Query DataSets for GSE112939
Status Public on Jul 16, 2019
Title Myeloid Skewing in Aging Bone Marrow Depends on Long-lived Plasma Cell Accumulation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Aging results in a reduction in B lymphopoiesis and an increase in myelopoiesis in mice and humans. We investigated how cellular changes in the aging hematopoietic microenvironment contribute to these alterations in hematopoiesis and found that long-lived plasma cells (LLPCs) increase in number in the bone marrow of old mice. The LLPCs exhibited a Toll Like Receptor activation signal, and their accumulation was partially dependent on inflammatory cytokines. LLPCs were able to stimulate myelopoiesis from hematopoietic stem cells and myeloid progenitors in vitro, and anti-CD138 antibody-mediated depletion of LLPCs or blockade of pro-inflammatory signaling in old mice in vivo resulted in a significant reduction in LLPC number and attenuated myeloid development. However, B lymphopoiesis remained suppressed. These data identify the increase in LLPC number as obligate for age-associated increases in myelopoiesis and provide evidence that, in the context of aging, enhanced myelopoiesis and depressed lymphopoiesis are distinct, independently regulated processes.
Overall design We isolated bone marrow long-lived plasma cells (LLPCs) as CD45R(B220)- IgD- CD138high TCRb- TCRgd- CD11b- Gr-1- from three independent groups of young (2-3 months, female) and old (17-19 months, male) mice and performed RNA-Seq.
Contributor(s) Pioli PD, Montecino-Rodriguez E, Casero D, Morrison SL, Dorshkind K
Citation(s) 31303400
Submission date Apr 10, 2018
Last update date Jul 16, 2019
Contact name Kenneth Dorshkind
Phone 310 206-9535
Organization name David Geffen School of Medicine at UCLA
Department Pathology and Laboratory Medicine
Street address 10833 Le Conte Avenue
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM3091643 Young LLPC #1
GSM3091644 Young LLPC #2
GSM3091645 Young LLPC #3
BioProject PRJNA449533

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112939_LLPC_genesfpkms.txt.gz 447.3 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data are available on Series record

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