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Series GSE11329 Query DataSets for GSE11329
Status Public on May 06, 2008
Title Chip-chip from mouse embryonic stem (mES) cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary To understand how pluripotency is controlled, we employed a modification of standard ChIP-chip method (biotin-mediated ChIP-chip) to identify targets of nine factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1) in mouse ES cells.
We show the application of In vivo biotinylation mediated ChIP (bioChIP) to global target mapping. Our data also show that differential regulation of target genes correlates with the extent of promoter occupancy by multiple factors in maintaining stem cell pluripotency.
Keywords: Biotin-mediated Chip-chip, Antibody ChIP-chip, Mouse embryonic stem cells
Overall design Comparison of targets of nine transcription factors in mES cells
Contributor(s) Kim J, Chu J, Shen X, Wang J, Orkin SH
Citation(s) 18358816
Submission date May 02, 2008
Last update date May 11, 2018
Contact name Jonghwan Kim
Phone 607-919-2051
Organization name HMS/CHB
Lab Stuart H Orkin lab
Street address 1 Blackfan circle Karp-7
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL5811 [Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array
Samples (14)
GSM286118 bioNanog
GSM286119 bioDax1
GSM286120 bioNac1
BioProject PRJNA106653

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11329_RAW.tar 5.1 Gb (http)(custom) TAR (of BAR, BED, CEL)
Processed data provided as supplementary file

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