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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 06, 2008 |
| Title |
Chip-chip from mouse embryonic stem (mES) cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
To understand how pluripotency is controlled, we employed a modification of standard ChIP-chip method (biotin-mediated ChIP-chip) to identify targets of nine factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1) in mouse ES cells.
We show the application of In vivo biotinylation mediated ChIP (bioChIP) to global target mapping. Our data also show that differential regulation of target genes correlates with the extent of promoter occupancy by multiple factors in maintaining stem cell pluripotency.
Keywords: Biotin-mediated Chip-chip, Antibody ChIP-chip, Mouse embryonic stem cells
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| Overall design |
Comparison of targets of nine transcription factors in mES cells
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| Contributor(s) |
Kim J, Chu J, Shen X, Wang J, Orkin SH |
| Citation(s) |
18358816 |
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| Submission date |
May 02, 2008 |
| Last update date |
May 11, 2018 |
| Contact name |
Jonghwan Kim |
| E-mail(s) |
jkim@bloodgroup.tch.harvard.edu, jybella@yahoo.com
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| Phone |
607-919-2051
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| Organization name |
HMS/CHB
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| Lab |
Stuart H Orkin lab
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| Street address |
1 Blackfan circle Karp-7
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA106653 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11329_RAW.tar |
5.1 Gb |
(http)(custom) |
TAR (of BAR, BED, CEL) |
| Processed data provided as supplementary file |
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