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Series GSE113293 Query DataSets for GSE113293
Status Public on Jun 30, 2019
Title Phenotyping spermatogenic defects by single-cell expression profiling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using Drop-seq, we generated high-throughput single-cell expression data from wild-type and four mutant models with male infertility phenotype. Our study demonstrates the applicability of single-cell RNA-sequencing in study of male gonadal dysfunction and provides cell atlas resource for testis.
Overall design Drop-seq was performed on FACS sorted germ cell populations, wild-type whole testes and mutant whole testes. Different experimental batches for wild-type and mutant strains were generated.
Contributor(s) Jung M, Conrad D
Citation(s) 31237565
Submission date Apr 17, 2018
Last update date Apr 29, 2022
Contact name Donald F. Conrad
Organization name Oregon Health & Science University
Department Division of Genetics; Oregon National Primate Research Center
Street address 505 NW 185th Ave
City Beaverton
State/province OR
ZIP/Postal code 97006
Country USA
Platforms (3)
GPL16417 Illumina MiSeq (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (27)
GSM3102982 Spermatogonia
GSM3102983 Primary Spermatocyte
GSM3102984 Secondary Spermatocyte
BioProject PRJNA450677
SRA SRP140739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113293_RAW.tar 58.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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