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| Status |
Public on Feb 28, 2019 |
| Title |
Exosomal lncRNA sequencing in Atorvastatin treated and non-treated MSC-derived exosomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In order to investigate the molecular mechanisms underlying the further enhancement of Atorvastatin pretreated MSC-derived exosomes (MSCATV-Exo) in cardiac protection, we performed lncRNA sequencing on exosomes secreted from ATV pretreated MSCs and non treated MSCs to identify differentially expressed lncRNA. We found that 450 lncRNAs were identified to be upregulated and 1332 lncRNAs downregulated (over 1.5 fold change) in MSCATV-Exo compared to MSC-Exo.
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| Overall design |
Two samples were processed for each kind of exosomes that derived from normal MSCs and ATV pretreating MSCs (MSC-Exo and MSCATV-Exo). MSCATV-Exo was isolated from conditioned medium of MSCs pretreated by 1μmol/L of ATV for 24 hours. RNA isolated from exosomes were analyzed using Illumina HiSeq 2500.
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| |
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| Contributor(s) |
Huang P, Yang Y |
| Citation(s) |
31119268 |
| |
| Submission date |
Apr 24, 2018 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Peisen Huang |
| E-mail(s) |
hps0814@qq.com
|
| Phone |
4043844891
|
| Organization name |
Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
|
| Street address |
No.167 Bei Li Shi Road, Xicheng District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100037 |
| Country |
China |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (4)
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| GSM3109212 |
MSC-Exo-1: exosomes derived from normal MSCs |
| GSM3109213 |
MSC-Exo-2: exosomes derived from normal MSCs |
| GSM3109214 |
MSCATV-Exo-1: exosomes derived from ATV pretreated MSCs |
| GSM3109215 |
MSCATV-Exo-2: exosomes derived from ATV pretreated MSCs |
|
| Relations |
| BioProject |
PRJNA453127 |
| SRA |
SRP142436 |
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