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| Status |
Public on Oct 01, 2019 |
| Title |
Extensive Transcriptional and Translational Regulation Occur During the Maturation of Malaria Parasite Sporozoites |
| Organisms |
Plasmodium falciparum 3D7; Plasmodium yoelii yoelii 17XNL |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
These data were used to assess gene expression and dynamics between oocyst sporozoites and salivary gland sporozoites for both Plasmodium falciparum and Plasmodium yoelii.
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| Overall design |
Sporozoites (Plasmodium falciparum, Plasmodium yoelii) from either the oocyst or salivary gland of Anopheles stephensi mosquitoes were purified twice by an Accudenz discontinuous density gradient and their RNA was extracted using a Qiagen Rneasy Kit with added DNaseI module. All experiments were done in biological triplicate.
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| Contributor(s) |
Lindner SE, Swearingen KE, Shears M, Walker MP, Vrana EN, Hart KJ, Minns AM, Sinnis P, Moritz RL, Kappe SH |
| Citation(s) |
31673027 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K22 AI101039 |
Dissection of RNA Storage Granules Essential to Plasmodium Transmission |
PENNSYLVANIA STATE UNIVERSITY-UNIV PARK |
Scott Eugene Lindner |
| R01 AI123341 |
Mechanisms Governing Translational Regulation During Plasmodium Transmission |
PENNSYLVANIA STATE UNIVERSITY-UNIV PARK |
Scott Eugene Lindner |
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| Submission date |
Apr 24, 2018 |
| Last update date |
Nov 14, 2019 |
| Contact name |
Scott E. Lindner |
| E-mail(s) |
Scott.Lindner@psu.edu
|
| Phone |
8148674062
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| Organization name |
Penn State University
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| Department |
Biochemistry and Molecular Biology
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| Street address |
W224 Millennium Science Complex
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| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (2) |
| GPL23784 |
Illumina HiSeq 2500 (Plasmodium yoelii yoelii 17XNL) |
| GPL24926 |
Illumina HiSeq 2500 (Plasmodium falciparum 3D7) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA453153 |
| SRA |
SRP142460 |
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