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| Status |
Public on Apr 26, 2018 |
| Title |
Transcription-driven Chromatin Repression of Intragenic Promoters |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
The goal of this study is to investigate a chromatin-based mechanism that suppresses intragenic initiation of RNAPII transcription in A.thaliana. We demonstrate that RNAPII transcription across gene promoters represses their function in plants. The FACT histone chaperone complex is required for this repression mechanism by co-transcriptionally enforcing elevated levels of H3K4me1 at repressed intragenic promoters;
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| Overall design |
TSS-Seq was used to map the transcription initiation sites with single base resolution in A.thaliana seedlings (Col-0 and FACT-deficient genotypes). We also used RNA-Seq to quantify gene expression changes in the same genotypes;
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| Web link |
https://doi.org/10.1101/279414
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| |
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| Contributor(s) |
Nielsen M, Ard R, Leng X, Ivanov M, Kindgren P, Pelechano V, Marquardt S |
| Citation(s) |
30707695, 32444691, 34058980 |
| |
| Submission date |
Apr 25, 2018 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Maxim Ivanov |
| E-mail(s) |
maxim.ivanov@plen.ku.dk
|
| Organization name |
University of Copenhagen
|
| Department |
Dept. of Plant and Environmental Sciences
|
| Street address |
Thorvaldsensvej 40
|
| City |
Frederiksberg C |
| ZIP/Postal code |
1871 |
| Country |
Denmark |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA453570 |
| SRA |
SRP142625 |
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