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Series GSE113677 Query DataSets for GSE113677
Status Public on Apr 26, 2018
Title Transcription-driven Chromatin Repression of Intragenic Promoters
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study is to investigate a chromatin-based mechanism that suppresses intragenic initiation of RNAPII transcription in A.thaliana. We demonstrate that RNAPII transcription across gene promoters represses their function in plants. The FACT histone chaperone complex is required for this repression mechanism by co-transcriptionally enforcing elevated levels of H3K4me1 at repressed intragenic promoters;
Overall design TSS-Seq was used to map the transcription initiation sites with single base resolution in A.thaliana seedlings (Col-0 and FACT-deficient genotypes). We also used RNA-Seq to quantify gene expression changes in the same genotypes;
Web link
Contributor(s) Nielsen M, Ard R, Leng X, Ivanov M, Kindgren P, Pelechano V, Marquardt S
Citation(s) 30707695, 32444691, 34058980
Submission date Apr 25, 2018
Last update date Jun 09, 2021
Contact name Maxim Ivanov
Organization name University of Copenhagen
Department Dept. of Plant and Environmental Sciences
Street address Thorvaldsensvej 40
City Frederiksberg C
ZIP/Postal code 1871
Country Denmark
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (9)
GSM3110981 Col0_TSS-Seq_rep1
GSM3110982 Col0_TSS-Seq_rep2
GSM3110983 spt16_TSS-Seq_rep1
BioProject PRJNA453570
SRA SRP142625

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113677_RAW.tar 261.4 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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