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Series GSE11373 Query DataSets for GSE11373
Status Public on May 08, 2008
Title Gene expression profile analysis for the Fascin knockdown esophageal squamous carcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Fascin, an actin-binding protein, is upregulated in a variety of cancers, including esophageal squamous cell carcinoma (ESCC) and is proposed to function in cellular growth, mobility and invasiveness. Nonetheless, the molecular mechanisms through which fascin expression contributes to the proliferation and invasiveness of ESCC are unknown.
To address this issue and to identify genes and biological pathways associated with fascin expression, a genome-wide gene expression analysis was performed on a fascin RNAi-depleted cell line and a corresponding ESCC cell line previously determined to express high levels of fascin.
Keywords: cell type comparison
Overall design We used cDNA microarrays to identify genes that were differentially expressed upon fascin knockdown. For this purpose, we selected PSF8 (Fascin siRNA-depleted cell) and PSC(blank vector control cell ), two ESCC cell lines recently established in our laboratory. Total RNA from PSF8 cells and PSC cells was prepared for use with Affymetrix U133 cDNA microarrays.
Contributor(s) Xie J, Xu L, Li E
Citation(s) 20056838
Submission date May 07, 2008
Last update date Mar 25, 2019
Contact name Enmin Li
Phone 86-754-8900847
Organization name Shantou university, medical college
Street address 22 Xinling Road
City shantou
State/province guangdong
ZIP/Postal code 515041
Country China
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (2)
GSM287274 PSC, vector control, biological rep1
GSM287275 PSF8, Fascin-knockdown, biological rep1
BioProject PRJNA106599

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Supplementary file Size Download File type/resource
GSE11373_RAW.tar 15.1 Mb (http)(custom) TAR (of CEL, EXP)
Processed data included within Sample table

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