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| Status |
Public on Oct 30, 2018 |
| Title |
Small RNA non-coding landscape of extracellular vesicles from human stem cells |
| Organisms |
Homo sapiens; other sequences |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In this study, we used RNA sequencing to provide a comprehensive overview of the expression profiles of small non-coding transcripts carried by the extracellular vesicles (EVs) derived from human adipose tissue stromal/stem cells (AT-MSCs) and human pluripotent stem cells (hPSCs), both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Small non-coding RNA sequencing from EVs showed that the profile of miRNA expression in PSC follow the profile reported for cell derived miRNA; further, most abundant miRNAs were found to originate from specific miRNA families which are regulating pluripotency, reprograming and differentiation (miR-17–92, mir-200, miR-302/367, miR-371/373, CM19 microRNA cluster). For the AT-MSCs, the highly expressed miRNAs were found to be regulating osteogenesis (mir-let-7/98, miR-10/100, miR-125, miR-196, miR-199, miR-615-3p, mir-22-3p, mir-24-3p, mir-27a-3p, mir-193b-5p, mir-195-3p). Additionally, abundant small nuclear and nucleolar RNA were detected in PSCs, whereas Y- and tRNA were found in AT-MSCs. Identification of EV-miRNA and non-coding RNA signatures released by these stem cells will provide clues towards understanding their role in intracellular communications, and well as their roles in maintaining the stem cell niche.
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| Overall design |
Small non-coding RNA profiles of extracellular vesicles derived from adipose tissue stromal/stem cells (AT-MSCs) and human pluripotent stem cells (hPSCs) generated by next-generation sequencing.
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| Contributor(s) |
Kaur S, Abu-Shahba AG, Paananen RO, Hiidenmaa H, Hongisto H, Skottman H, Seppänen-Kaijansinkko R, Mannerström B |
| Citation(s) |
30341351 |
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| Submission date |
Apr 30, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Sippy Kaur |
| E-mail(s) |
sippy.kaur@helsinki.fi
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| Organization name |
University of Helsinki
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| Department |
Oral and Maxillofacial Diseases
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| Street address |
PO Box 63
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| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24755 |
Illumina NextSeq 500 (other sequences) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA454305 |
| SRA |
SRP144059 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113868_mirna_raw_counts.txt.gz |
32.3 Kb |
(ftp)(http) |
TXT |
| GSE113868_smrna_raw_counts.txt.gz |
110.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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