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Series GSE113894 Query DataSets for GSE113894
Status Public on May 04, 2018
Title The KNOXI gene SHOOT MERISTEMLESS regulates floral fate in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plants have evolved a unique and conserved developmental program that enables the conversion of leaves into floral organs. Elegant genetic and molecular work has identified key regulators of floral meristem identity. However, further understanding of flower meristem specification has been hampered by redundancy and by pleiotropic effects. The KNOXI gene STM transcription factor is a well-characterized regulator of shoot apical meristem maintenance. stm loss-of-function mutants arrest shortly after germination, and therefore the knowledge on later roles of STM, including flower development, is limited. Here, we uncover a role for STM in the specification of flower meristem identity. Silencing STM in the AP1 expression domain in the ap1-4 mutant background resulted in a complete leafy-like flower phenotype and an intermediate stm-2 allele enhanced the floral meristem identity phenotype of ap1-4. Transcriptional profiling of STM perturbation suggested that STM activity affects multiple meristem identity and flower transition genes, among them the F-Box gene UFO. In agreement, stm-2 enhanced the ufo-2 floral meristem fate phenotype, and ectopic UFO expression rescued the leafy flowers in genetic backgrounds with compromised AP1 and STM activities. This work suggests a molecular mechanism that underlies the activity of STM in the specification of flower meristem identity.
 
Overall design Silencing STM in the AP1 expression domain in the ap1-4 mutant
 
Contributor(s) Roth O, Shani E
Citation(s) 29743198
Submission date May 01, 2018
Last update date Sep 20, 2018
Contact name Eilon Shani
E-mail(s) eilonsh@post.tau.ac.il
Organization name TAU
Street address Ramat Aviv
City Tel Aviv
ZIP/Postal code 6997801
Country Israel
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (15)
GSM3123067 ap1 rep1
GSM3123068 ap1 rep2
GSM3123069 ap1 rep3
Relations
BioProject PRJNA454379
SRA SRP144164

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113894_Normalized_Counts_AT_STM.txt.gz 2.6 Mb (ftp)(http) TXT
GSE113894_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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