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Status |
Public on Dec 21, 2018 |
Title |
Heavy chain immunoglobulin sequencing of Lyme disease PBMCs |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
Lyme disease (Borrelia burgdorferi infection) is increasingly recognized as a significant source of morbidity world-wide. Here, we investigated B cell responses to Lyme disease through molecular identifier-enabled antibody heavy chain sequencing of bulk B cells from PBMCs. Single-cell immunoglobulin sequencing of paired heavy- and light-chain genes from this project will also be separately deposited. Additional information regarding patient characteristics and overlap with other data from the SLICE study is available upon request.
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Overall design |
Total mRNA was isolated from peripheral blood mononuclear cells (PBMCs) of Lyme disease patients at four timepoints: Visit 1, Untreated acute infection; Visit 3, one month after completion of treatment; Visit 5, six months after treatment; Visit 7, two years after treatment. Total mRNA was isolated from peripheral blood mononuclear cells (PBMCs) of Healthy controls at three timepoints: Visit H2, initial visit; Visit H3, six months after initial; Visit H4, one year after initial visit. cDNA was tagged with unique molecular identifiers, amplified with primers specific for immunoglobulin constant regions, and sequenced by HiSeq 2500.
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Contributor(s) |
Blum L, Robinson WH, Rebman A |
Citation(s) |
30072990 |
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Submission date |
May 01, 2018 |
Last update date |
Mar 27, 2019 |
Contact name |
Lisa K Blum |
E-mail(s) |
lkblum@gmail.com
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Phone |
805-234-7162
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Organization name |
Stanford University
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Lab |
William H. Robinson
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Street address |
269 Campus Drive
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City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (71)
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This SubSeries is part of SuperSeries: |
GSE114310 |
Immunoglobulin sequencing of Lyme disease individuals |
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Relations |
BioProject |
PRJNA454501 |
SRA |
SRP144230 |