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Series GSE114042 Query DataSets for GSE114042
Status Public on Oct 09, 2019
Title RNA-seq of 10dpp wild type and Dazl SKO mouse testes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we generated conditional DAZL knockout mice which allowed us to examine the roles of DAZL in postnatal spermatogenesis without affecting its early function in PGC. RNA was extracted from 10dpp wild type and Stra8-cre KO mouse testes to perform RNA-seq, StringTie software was used to assess the expression level by formula log2(FPKM+1).
 
Overall design RNA-seq of 10dpp wild type and Dazl SKO mouse testes
 
Contributor(s) Xu EY, Li H
Citation(s) 31355046
Submission date May 04, 2018
Last update date Oct 09, 2019
Contact name Haixin Li
E-mail(s) lihaixin@njmu.edu.cn
Organization name Nanjing Medical University
Department State Key Laboratory of Reproductive Medicine
Street address 101 Longmian Rd
City Nanjing
State/province Jiangsu
ZIP/Postal code 211166
Country China
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (2)
GSM3131276 10dpp wild type mouse testes
GSM3131277 10dpp Dazl SKO mouse testes
Relations
BioProject PRJNA462763
SRA SRP144618

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114042_Gene_expression_StringTie_log2_FPKM+1_.xlsx 1.6 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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