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Status |
Public on Oct 09, 2019 |
Title |
RNA-seq of 10dpp wild type and Dazl SKO mouse testes |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The autosomal gene Dazl is a member of highly conservative DAZ family, all of which contains a RNA binding domain and expresses in germ cells. In mice, Dazl plays an essential role in germ cell development, germ cell lost in Dazl knockout mouse on pure C57BL/6 strain background. Here we generated conditional DAZL knockout mice which allowed us to examine the roles of DAZL in postnatal spermatogenesis without affecting its early function in PGC. RNA was extracted from 10dpp wild type and Stra8-cre KO mouse testes to perform RNA-seq, StringTie software was used to assess the expression level by formula log2(FPKM+1).
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Overall design |
RNA-seq of 10dpp wild type and Dazl SKO mouse testes
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Contributor(s) |
Xu EY, Li H |
Citation(s) |
31355046 |
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Submission date |
May 04, 2018 |
Last update date |
Oct 09, 2019 |
Contact name |
Haixin Li |
E-mail(s) |
lihaixin@njmu.edu.cn
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Organization name |
Nanjing Medical University
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Department |
State Key Laboratory of Reproductive Medicine
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Street address |
101 Longmian Rd
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City |
Nanjing |
State/province |
Jiangsu |
ZIP/Postal code |
211166 |
Country |
China |
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Platforms (1) |
GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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Samples (2) |
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Relations |
BioProject |
PRJNA462763 |
SRA |
SRP144618 |