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Series GSE114333 Query DataSets for GSE114333
Status Public on Feb 13, 2019
Title DNA de novo (de)Methylation in the Kidney Contributes to Salt-Induced Hypertension [RRBS-seq]
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary Numerous adult diseases involving tissues that consist primarily of non-dividing cells are associated with changes in DNA methylation. It suggests a role for de novo methylation or demethylation of DNA, which is catalyzed by DNA methyltransferase 3 (Dnmt3) and ten-eleven translocases (Tet). However, the contribution of DNA de novo (de)methylation to these diseases remains nearly completely unproven. Broad changes in DNA methylation occurred within days in the renal outer medulla of Dahl SS rats fed a high-salt diet, a classic model of hypertension. Intra-renal administration of anti-Dnmt3a/Tet3 GapmeR’s attenuated high salt-induced hypertension in SS rats. The high salt diet induced differential expression of 1,712 genes in the renal outer medulla. Remarkably, the differential expression of 76% of these genes were prevented by anti-Dnmt3a/Tet3 GapmeR’s. The genes differentially expressed in response to the GapmeR’s were involved in the regulation of metabolism and inflammation and were significantly enriched for genes showing differential methylation in response to the GapmeR’s. These data indicate DNA de novo (de)methylation in the kidney contributes to the development of hypertension in SS rats. The findings should help to shift the paradigm of DNA methylation research in diseases involving non-dividing cells from correlative analysis to functional and mechanistic studies.
 
Overall design DNA methylation profile analysis was performed in renal outer medulla tissues from individual rats (7 groups, n=3-4 per group) using multiplexed RRBS protocols
 
Contributor(s) Liang M, Liu P
Citation(s) 30354815
Submission date May 10, 2018
Last update date Feb 13, 2019
Contact name Pengyuan Liu
E-mail(s) pliu@mcw.edu
Phone 4149552833
Organization name Medical College of Wisconsin
Department Physiology
Street address 8701 Watertown Plank Road
City Milwaukee
State/province Wisconsin
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (27)
GSM3139698 MCW SS LS Replicate 1 [1155_RP]
GSM3139699 MCW SS LS Replicate 2 [1156]
GSM3139700 MCW SS LS Replicate 3 [1245]
This SubSeries is part of SuperSeries:
GSE114335 DNA de novo (de)Methylation in the Kidney Contributes to Salt-Induced Hypertension
Relations
BioProject PRJNA470984
SRA SRP145417

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114333_RRBS.dataset1.processed.txt.gz 41.9 Mb (ftp)(http) TXT
GSE114333_RRBS.dataset2.processed.txt.gz 68.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
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