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Series GSE114356 Query DataSets for GSE114356
Status Public on Feb 13, 2019
Title This study examined gene expression in zebrafish embryos exposed to 0 (0.01% DMSO), 16 µM, or 32 µM perfluorobutane sulfonate.
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary The objective of this study is to compare the toxicity of the emerging replacement surfactant PFBS to the legacy chemical PFOS in the developing pancreas. Because of its chemical properties, it is hypothesized that PFBS would result in fewer or more mild aberrant pancreas phenotypes and ameliorated pancreas gene expression compared to PFOS. Herein, we use transgenic zebrafish models to visualize these processes in vivo throughout the developmental process, and use RNA-Seq to examine major pathways affected by these embryonic exposures.
Overall design Each sample contained 15 pooled zebrafish embryos at 96 hours post fertilization. Three samples were analyzed per exposure group (n=3 samples for each exposure). Three experimental replicates were performed, each yielding one sample per exposure group. Embryos were exposed in 100 mm polystyrene plates containing 30 ml of exposure media, prepared in 0.3x Danieau's medium. Prior to collection, embryos were thoroughly washed 3 times to remove excess PFBS.
Contributor(s) Sant KE, Sinno PP, Venezia OL, Timme-Laragy AR
Citation(s) 30239974
Submission date May 11, 2018
Last update date Feb 13, 2019
Contact name Karilyn Sant
Organization name San Diego State University
Department School of Public Health
Lab Sant
Street address 5500 Campanille Dr., Hardy Tower 119
City San Diego
State/province CA
ZIP/Postal code 92182
Country USA
Platforms (1)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
Samples (9)
GSM3140412 KS1-S1
GSM3140413 KS2-S2
GSM3140414 KS3-S3
BioProject PRJNA471075
SRA SRP145495

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114356_Control-PFBS16uM.csv.gz 605.6 Kb (ftp)(http) CSV
GSE114356_Control-PFBS32uM.csv.gz 643.1 Kb (ftp)(http) CSV
GSE114356_PFBS16uM-PFBS32uM.csv.gz 605.2 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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