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Series GSE11437 Query DataSets for GSE11437
Status Public on May 22, 2008
Title Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array
Platform organism Toxoplasma gondii
Sample organisms Toxoplasma gondii; synthetic construct
Experiment type Expression profiling by array
Summary Toxoplasma gondii is an intracellular parasite with a significant impact on human health, especially in cases where individuals are immunocompromised (e.g., due to HIV/AIDS). In Europe and North America only a few clonal genotypes appear to be responsible for the vast majority of Toxoplasma infections, and these clonotypes have been intensely studied to identify strain-specific phenotypes that may play a role in the manifestation of more severe disease. To identify and genetically map strain-specific differences in gene expression, we have carried out expression quantitative trait locus (eQTL) analysis on Toxoplasma gene expression phenotypes using spotted cDNA microarrays. This led to the identification of 16 Toxoplasma genes that had significant and mappable strain-specific variation in hybridization intensity. While the analysis should identify both cis and trans-mapping hybridization profiles, we only identified loci with strain-specific hybridization differences that are most likely due to differences in the locus itself (i.e., cis-mapping). Interestingly, a larger number of these cis-mapping genes than would be expected by chance encode either confirmed or predicted secreted proteins, many of which are known to localize to the specialized secretory organelles characteristic of members of the phylum Apicomplexa. For 6 of the cis-mapping loci we determined if the strain-specific hybridization differences were due to true transcriptional differences or rather strain-specific differences in hybridization efficiency because of extreme polymorphism and/or deletion, and we found examples of both scenarios.
Keywords: eQTL mapping; virulence; Toxoplasma gondii
Overall design 17 F1 progeny from a cross between a type II parent (PDS) and a type III parent (CTG) were used in RNA hybridizations to identify cis and trans-mapping loci regulating gene expression
Contributor(s) Boyle JP, Saeij JP, Boothroyd JC
Citation(s) 18552283
Submission date May 14, 2008
Last update date Mar 19, 2012
Contact name Jon Boyle
Organization name Stanford University School of Medicine
Department Microbiology and Immunology
Lab Boothroyd Lab
Street address 299 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 92305
Country USA
Platforms (1)
GPL6851 Boothroyd Lab Toxoplasma gondii bradyzoite 4K
Samples (24)
GSM288465 Strain CTG Tachyzoite_1
GSM288466 Strain CTG Tachyzoite_2
GSM288467 Strain CTG Tachyzoite_3
This SubSeries is part of SuperSeries:
GSE11515 Expression QTL mapping of Toxoplasma gondii genes
BioProject PRJNA109113

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11437_RAW.tar 27.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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