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| Status |
Public on May 22, 2008 |
| Title |
Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array |
| Platform organism |
Toxoplasma gondii |
| Sample organisms |
Toxoplasma gondii; synthetic construct |
| Experiment type |
Expression profiling by array
|
| Summary |
Toxoplasma gondii is an intracellular parasite with a significant impact on human health, especially in cases where individuals are immunocompromised (e.g., due to HIV/AIDS). In Europe and North America only a few clonal genotypes appear to be responsible for the vast majority of Toxoplasma infections, and these clonotypes have been intensely studied to identify strain-specific phenotypes that may play a role in the manifestation of more severe disease. To identify and genetically map strain-specific differences in gene expression, we have carried out expression quantitative trait locus (eQTL) analysis on Toxoplasma gene expression phenotypes using spotted cDNA microarrays. This led to the identification of 16 Toxoplasma genes that had significant and mappable strain-specific variation in hybridization intensity. While the analysis should identify both cis and trans-mapping hybridization profiles, we only identified loci with strain-specific hybridization differences that are most likely due to differences in the locus itself (i.e., cis-mapping). Interestingly, a larger number of these cis-mapping genes than would be expected by chance encode either confirmed or predicted secreted proteins, many of which are known to localize to the specialized secretory organelles characteristic of members of the phylum Apicomplexa. For 6 of the cis-mapping loci we determined if the strain-specific hybridization differences were due to true transcriptional differences or rather strain-specific differences in hybridization efficiency because of extreme polymorphism and/or deletion, and we found examples of both scenarios.
Keywords: eQTL mapping; virulence; Toxoplasma gondii
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| Overall design |
17 F1 progeny from a cross between a type II parent (PDS) and a type III parent (CTG) were used in RNA hybridizations to identify cis and trans-mapping loci regulating gene expression
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| Contributor(s) |
Boyle JP, Saeij JP, Boothroyd JC |
| Citation(s) |
18552283 |
| |
| Submission date |
May 14, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Jon Boyle |
| E-mail(s) |
boylej@stanford.edu
|
| Organization name |
Stanford University School of Medicine
|
| Department |
Microbiology and Immunology
|
| Lab |
Boothroyd Lab
|
| Street address |
299 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
92305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6851 |
Boothroyd Lab Toxoplasma gondii bradyzoite 4K |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE11515 |
Expression QTL mapping of Toxoplasma gondii genes |
|
| Relations |
| BioProject |
PRJNA109113 |
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