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| Status |
Public on Oct 03, 2018 |
| Title |
TRPS1 is a lineage-specific transcriptional dependency in breast cancer [methylation array] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
We performed an unbiased cell viability-based pooled shRNA screen on 59 cell lines to identify novel epigenetic and transcriptional dependencies of multiple cancer types, including leukemia, neuroblastoma, breast, colorectal, prostate, and rhabdoid tumors. Here, we identified Tricho-Rhino-Phalangeal Syndrome Type I protein (TRPS1) as one of the most significant hits specific for breast cancer cell lines. Downregulation of TRPS1 resulted in cell cycle arrest and apoptosis increase in vitro and impaired tumorigenic capacity in vivo. We characterized TRPS1 genomic targets and protein interactome. We identified GATAD2B as an important partner of TRPS1, uncovering novel epigenetic network crucial for breast cancer cell survival.
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| Overall design |
DNA methylation: Array based methylation analysis of 59 cell lines.
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| Contributor(s) |
Witwicki RM, Ekram MB, Qiu X, Janiszewska M, Shu S, Kwon M, Trinh A, Frias E, Cochran N, Hoffman G, Xie Y, McAllister G, McDonald R, Golji J, Schlabach M, Venkatesan K, deWeck A, Keen N, Chan HM, Ruddy D, Rejtar T, Sovath S, Silver S, Sellers W, Jagani Z, Hogarty MD, Shivdasani RA, Roberts C, Brown M, Stegmaier K, Long H, Pellman D, Polyak K |
| Citation(s) |
30380416 |
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| Submission date |
May 17, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Kornelia Polyak |
| E-mail(s) |
kornelia_polyak@dfci.harvard.edu
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| Phone |
617-632-2106
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| Organization name |
Dana-Farber Cancer Institute
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| Department |
Medical Oncology
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| Lab |
Polyak
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| Street address |
450 Brookline Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (61)
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| This SubSeries is part of SuperSeries: |
| GSE114600 |
TRPS1 is a lineage-specific transcriptional dependency in breast cancer |
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| Relations |
| BioProject |
PRJNA471882 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE114598_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE114598_raw_data.txt.gz |
138.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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