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Series GSE114640 Query DataSets for GSE114640
Status Public on Jul 30, 2020
Title Mouse embryonic stem cells (mESCs) differentiation towards ventral hindbrain neuronal cell types [d4.5, d5.5 and d6.5]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary mESCs treated with retinoic acid (RA) and a Shh agonist (SAG) recapitulated ventral hindbrain development and produced a population of neural progenitors with a minor presence of other cell types.
 
Overall design The differentiation in mESCs was induced by RA and SAG in monolayer condition. The sorted cells were collected at different timepoints (i.e., d4.5, d5.5, d6.5) and processed for RNA isolation and RNA-seq.
 
Contributor(s) Ericson J, Alekseenko Z
Citation(s) 32938678
Submission date May 18, 2018
Last update date Oct 29, 2020
Contact name Johan Ericson
E-mail(s) johan.ericson@ki.se
Organization name Karolinska Instituet
Department Cell and Molecular Biology
Lab Johan Ericson's Group
Street address Solnav├Ągen 9
City Stockholm
ZIP/Postal code 171 65
Country Sweden
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM3081049 WT_prog_4.5_HB_rep1
GSM3081050 WT_prog_4.5_HB_rep2
GSM3081051 WT_prog_4.5_HB_rep3
Relations
BioProject PRJNA472041
SRA SRP188856

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114640_Temporal_WT_Counts.txt.gz 401.6 Kb (ftp)(http) TXT
GSE114640_Temporal_WT_Voomed.txt.gz 1.2 Mb (ftp)(http) TXT
GSE114640_Temporal_WT_fpkms.txt.gz 858.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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