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| Status |
Public on Jul 30, 2020 |
| Title |
Mouse embryonic stem cells (mESCs) differentiation towards ventral hindbrain neuronal cell types [d4.5, d5.5 and d6.5] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
mESCs treated with retinoic acid (RA) and a Shh agonist (SAG) recapitulated ventral hindbrain development and produced a population of neural progenitors with a minor presence of other cell types.
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| Overall design |
The differentiation in mESCs was induced by RA and SAG in monolayer condition. The sorted cells were collected at different timepoints (i.e., d4.5, d5.5, d6.5) and processed for RNA isolation and RNA-seq.
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| |
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| Contributor(s) |
Ericson J, Alekseenko Z |
| Citation(s) |
32938678 |
| |
| Submission date |
May 18, 2018 |
| Last update date |
Oct 29, 2020 |
| Contact name |
Johan Ericson |
| E-mail(s) |
johan.ericson@ki.se
|
| Organization name |
Karolinska Instituet
|
| Department |
Cell and Molecular Biology
|
| Lab |
Johan Ericson's Group
|
| Street address |
Solnavägen 9
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA472041 |
| SRA |
SRP188856 |
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