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Series GSE114696 Query DataSets for GSE114696
Status Public on Nov 19, 2018
Title Plant Extracellular Vesicles Contain Diverse Small RNA Species and Are Enriched in 10 to 17 Nucleotide "Tiny" RNAs.
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Small RNAs (sRNAs) are small non-coding RNAs of 20 to 24 nucleotides in size found across a wide range of eukaryote organisms, from plants to animals. sRNAs are master regulators of a wide range of biological functions, including development, reproduction and stress responses. They typically function via the control of the stability and translation of target mRNAs. sRNAs can be divided in two major classes, according to their biogenesis and mode of action: microRNAs (miRNAs) and small interfering RNAs (siRNAs). As a silencing signal, several different types of sRNAs have also the ability to move short distances, like cell-to-cell, or much longer distances like root to shoot. They are even trafficked between different species and organisms. However, the mechanisms by which sRNAs move remain unknown. Extracellular vesicles (EVs) are mobile lipid compartments that participate in intercellular communication; they are able to carry proteins, lipids, nucleic acids and metabolites. Despite the fact that very little is known about the origin, formation or function of EVs in plants, recent results suggest that they participate in both long distance movement and immune responses. In this study, we analysed the small RNA content of EVs, and we identified miRNAs and siRNAs that are preferentially loaded into the EVs. This EV enrichment of certain RNAs supports hypotheses for long-distance RNA movement and for RNA-based communication between species, including host-pathogen interactions.
Overall design [Col-0] 3 different biological replicates of small RNA seq from Arabidopsis leaf total RNA, Extracellular vesicles and supernatant are included in this study.
[dcl234 triple mutant] 2 different biological replicates of small RNA seq from dcl234 triple mutant Arabidopsis leaf total RNA, Extracellular vesicles and supernatant are included in this study.
Contributor(s) Baldrich P, Rutter BD, Podicheti R, Innes RW, Meyers BC
Citation(s) 30705133
Submission date May 21, 2018
Last update date Mar 19, 2019
Contact name Patricia Baldrich
Organization name Donald Danforth Plant Science Center
Department Meyers Lab
Lab Meyers Lab
Street address 975 N warson Road
City Saint Louis
State/province MO
ZIP/Postal code 63132
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (15)
GSM3147536 Leaf TotalRNA, Col-0, Replicate 1
GSM3147537 Leaf TotalRNA, Col-0, Replicate 2
GSM3147538 Leaf TotalRNA, Col-0, Replicate 3
BioProject PRJNA472309
SRA SRP148558

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Supplementary file Size Download File type/resource
GSE114696_RAW.tar 420.9 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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