GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE114723 Query DataSets for GSE114723
Status Public on Nov 03, 2018
Title Spatial Transcriptomics of C. elegans Males and Hermaphrodites Identifies Sex-Specific Differences in Gene Expression Patterns
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial
transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes.
We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns.
Overall design Spatial transcriptomics by RNA-tomography (TomoSeq) on 20um sections
Web link
Contributor(s) Ebbing A, Vertesy A, Betist M, Spanjaard B, Junker JP, Berezikov E, van Oudenaarden A, Korswagen HC
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 21, 2018
Last update date Nov 05, 2018
Contact name Abel Vertesy
Organization name Hubrecht Institute
Lab Alexander van Oudenaarden
Street address Uppsalalaan 8
City Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
Platforms (1)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
Samples (10)
GSM3148565 glp-1 mutant animal nr.1
GSM3148566 glp-1 mutant animal nr.2
GSM3148567 Male animal nr.1
BioProject PRJNA472377
SRA SRP148595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114723_CEL-seq.1.demultiplexing.barcodes.pdf 63.1 Kb (ftp)(http) PDF
GSE114723_RAW.tar 13.2 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap