GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE114919 Query DataSets for GSE114919
Status Public on Jun 19, 2018
Title Differential ageing of growth plate cartilage determines skeletal proportions
Organisms Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Bones at different anatomical locations vary dramatically in size. The mechanisms responsible for these size differences are poorly understood. Bone elongation occurs at the growth plates and advances rapidly in early life but then progressively slows due to a developmental program termed growth plate senescence. This developmental program includes declines in cell proliferation and hypertrophy, depletion of cells in all growth plate zones, and extensive underlying changes in the expression of growth-regulating genes. Here we use RNA-Seq to compare changes of gene expression with age in the longer bone (tibia, 1- vs 4-wk) with the difference of gene expression between long and short bones (tibia vs phalanx) at 1wk. We found that the developmental program of growth plate senescence is more advanced in the shorter bone and this differential senescence (or aging) underlies the disparities in bone length.
Overall design tibia, 1wk vs 4wk; 1wk, tibia vs phalanx; in both mice and rats, proliferative zone or hypertrophic zone of growth plate (separated by laser capture microdissection)
Contributor(s) Lui J, Baron J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date May 25, 2018
Last update date Feb 11, 2019
Contact name Julian CK Lui
Phone 301-496-8049
Organization name NICHD, NIH
Lab Section on Growth and Development
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
GPL23945 Illumina HiSeq 3000 (Rattus norvegicus)
Samples (60)
GSM3154535 mouse 1wk tibia HZ 1
GSM3154536 mouse 1wk tibia HZ 2
GSM3154537 mouse 1wk tibia HZ 3
BioProject PRJNA473132
SRA SRP148968

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114919_Mouse_RNA-Seq_names.xlsx 12.0 Kb (ftp)(http) XLSX
GSE114919_Mouse_normalizedcounts.xlsx 6.6 Mb (ftp)(http) XLSX
GSE114919_Rat_RNA-Seq_names.xlsx 40.6 Kb (ftp)(http) XLSX
GSE114919_Rat_normalizedcounts.xlsx 6.1 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap