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Series GSE114985 Query DataSets for GSE114985
Status Public on May 14, 2019
Title The transgenerational effects of mitochondrial supplementation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the outcomes of offspring produced from mitochondrial supplementation to mature oocytes at the time of fertilisation, we generated three generations of offspring. Primordial follicles of the third generation female mice (postnatal day 3) were collected, and RNA sequencing was performed. We compared the treated and control gene expression data to determine differentially expressed genes between the two groups. Our data suggest that mitochondrial supplementation has transgenerational effects on offspring.
Overall design 1. mitochondrial supplementation at the time of fertilisation was performed 2. three generations of offspring were produced to determine the transgeneration effects of the technique. 3. Primordial follicles of the third generation day 3 postnatal offspring were collected and extracted to compare the transcripts of treated vs control primordial follicles.
Contributor(s) St. John J
Citation(s) 31040316
Submission date May 29, 2018
Last update date May 14, 2019
Contact name Justin C St. John
Phone +61399024749
Organization name Hudson Institute of Medical Research
Department Centre for Genetic Diseases
Lab Mitochondrial Genetics
Street address 27-31 Wright St
City Clayton
State/province Vic
ZIP/Postal code 3168
Country Australia
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (8)
GSM3161963 Biological Replicate 1_control (280_4)
GSM3161964 Biological Replicate 2_control (280_6)
GSM3161965 Biological Replicate 3_control (301_4)
BioProject PRJNA473442
SRA SRP149128

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Supplementary file Size Download File type/resource
GSE114985_gene_counts_8samples.txt.gz 305.3 Kb (ftp)(http) TXT
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