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Series GSE115094 Query DataSets for GSE115094
Status Public on Aug 07, 2018
Title Casp8- and Casp11-dependent gene expression in ileal mucosa of LPS-challenged male mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The execution of shock following high dose E. coli lipopolysaccharide (LPS) or bacterial sepsis in mice required pro-apoptotic caspase-8 in addition to pro-pyroptotic caspase-11 and gasdermin d. Hematopoietic cells produced MyD88- and TRIF-dependent inflammatory cytokines sufficient to initiate LPS shock independent of caspase-8 or caspase-11. Both proteases had to be present to support tissue injury, dependent upon TNF and interferon , first observed in the small intestine and later in spleen and thymus. Caspase-11 enhanced the activation of caspase-8 and extrinsic cell death machinery within the lower small intestine. Neither caspase-8 nor caspase-11 was individually sufficient for shock, but collaborated to amplify the inflammatory signals associated with tissue injury. Therefore, combined pyroptotic and apoptotic signaling mediated endotoxemia independent of RIPK1 kinase activity and RIPK3 function. These observations bring to light the relevance of tissue compartmentalization during inflammatory signaling in vivo where cytokines execute diverse cell death pathways and tissue damage.
Overall design Ileum was harvested from Wt (C57BL/6), Casp8-RIPK3-deficient and Casp11-deficient mice (5 male for each group) at 1.5 h post challenge with high dose LPS (54 mg/kg). Excised tissues were maintained in RNAlater solution for 24 h and RNA was harvested from mucosa.
Contributor(s) Mandal P, Mocarski ES, Bosinger SE, Patel N, Tharp GK
Citation(s) 30021146
Submission date May 30, 2018
Last update date Feb 11, 2019
Contact name Gregory K Tharp
Phone 404-727-7797
Organization name Yerkes National Primate Research Center
Department Developmental and Cognitive Neuroscience
Lab Genomics Core
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329-4208
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (15)
GSM3165062 1C-WT-1-LPS
GSM3165063 2C-WT-2-LPS
GSM3165064 3C-WT-3-LPS
BioProject PRJNA473781
SRA SRP149344

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Supplementary file Size Download File type/resource
GSE115094_293_Mandal_DESeq2_NormCounts.txt.gz 1.4 Mb (ftp)(http) TXT
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