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Series GSE115226 Query DataSets for GSE115226
Status Public on Nov 30, 2018
Title The HDAC3 Enzymatic Activity Regulates Skeletal Muscle Fuel Metabolism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We address whether the functions of HDAC3 in skeletal muscle require its enzyme activity. By mutating the NCoR/SMRT corepressors in a knock-in mouse model named NS-DADm, we ablated the enzymatic activity of HDAC3 without affecting its protein levels. Compared to the control mice, skeletal muscles from NS-DADm mice showed lower force generation, enhanced fatigue resistance, enhanced fatty acid oxidation, reduced glucose uptake during exercise, upregulated expression of metabolic genes involved in branched-chain amino acids (BCAAs) catabolism, and aging-associated reduction in muscle mass, without changes in the muscle fiber type composition or mitochondrial protein content. These findings demonstrate that the metabolic function of HDAC3 in skeletal muscles requires its enzymatic activity.
 
Overall design examination of 3 different samples in each genotype (WT and NS-DADm)
 
Contributor(s) Song S, Sun Z
Citation(s) 30428023
Submission date Jun 01, 2018
Last update date Mar 12, 2019
Contact name Shiyang Song
E-mail(s) shiyangsong1992@gmail.com
Phone 8322588569
Organization name Baylor College of Medicine
Department Medicine
Lab Zheng Sun's Lab
Street address 2111 Holly Hall ,Apt.2206
City Houston
State/province Texas
ZIP/Postal code 77054
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (6)
GSM3171686 WT1
GSM3171687 WT2
GSM3171688 WT3
Relations
BioProject PRJNA474203
SRA SRP149553

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE115226_processed_data.xlsx 153.5 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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