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Series GSE115403 Query DataSets for GSE115403
Status Public on Jul 01, 2019
Title Inflammation-produced TNFα protects hematopoietic stem cells from necroptosis and promotes hematopoietic regeneration
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To gain molecular insights into the differential effect of TNFα between hematopoietic stem cells (HSC) and downstream myeloid progenitors, we performed RNA-sequencing analyses of HSCs and granulocyte-macrophage progenitors (GMP) treated with TNFα both ex vivo and in vivo.
 
Overall design RNA sequencing of 3 biological replicates of HSCs (Lin-/cKit+/Sca1+/Flk2-/CD48-/CD150+) and GMPs (Lin-/cKit+/Sca1-/FcγR+/CD34+) treated ± TNFα both ex vivo for 3 and 12 hours and in vivo for 3 hour (1x3hr). For ex vivo treatment of TNFα, HSCs and GMPs were isolated by FACS from C57BL/6 mouse bone marrow (BM) and cultured in liquid media ± TNFα for either 3 or 12 hours. For in vivo treatment, HSCs and GMPs were isolated from C57BL/6 mice 3 hours after a single injection of PBS or TNFα.
 
Contributor(s) Passegué E, Yamashita M
Citation(s) 31230859
Submission date Jun 06, 2018
Last update date Jul 04, 2019
Contact name Masayuki Yamashita
Organization name Institute of Medical Science, University of Tokyo
Department Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine
Street address 4-6-1 Shirokanedai
City Minato-ku
State/province Tokyo
ZIP/Postal code 108-8639
Country Japan
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (36)
GSM3177641 ex 3hr in vivo -TNFα rep1
GSM3177642 ex 3hr in vivo -TNFα rep2
GSM3177643 ex 3hr in vivo -TNFα rep3
Relations
BioProject PRJNA474837
SRA SRP149892

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Supplementary file Size Download File type/resource
GSE115403_RAW.tar 11.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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