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| Status |
Public on Jul 01, 2019 |
| Title |
Inflammation-produced TNFα protects hematopoietic stem cells from necroptosis and promotes hematopoietic regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To gain molecular insights into the differential effect of TNFα between hematopoietic stem cells (HSC) and downstream myeloid progenitors, we performed RNA-sequencing analyses of HSCs and granulocyte-macrophage progenitors (GMP) treated with TNFα both ex vivo and in vivo.
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| Overall design |
RNA sequencing of 3 biological replicates of HSCs (Lin-/cKit+/Sca1+/Flk2-/CD48-/CD150+) and GMPs (Lin-/cKit+/Sca1-/FcγR+/CD34+) treated ± TNFα both ex vivo for 3 and 12 hours and in vivo for 3 hour (1x3hr). For ex vivo treatment of TNFα, HSCs and GMPs were isolated by FACS from C57BL/6 mouse bone marrow (BM) and cultured in liquid media ± TNFα for either 3 or 12 hours. For in vivo treatment, HSCs and GMPs were isolated from C57BL/6 mice 3 hours after a single injection of PBS or TNFα.
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| |
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| Contributor(s) |
Passegué E, Yamashita M |
| Citation(s) |
31230859 |
| |
| Submission date |
Jun 06, 2018 |
| Last update date |
Dec 16, 2024 |
| Contact name |
Masayuki Yamashita |
| E-mail(s) |
masayuki.yamashita@stjude.org
|
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Division of Experimental Hematology, Department of Hematology
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| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA474837 |
| SRA |
SRP149892 |
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