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| Status |
Public on Jul 30, 2018 |
| Title |
The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37 |
| Organism |
Clostridioides difficile |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Clostridium difficile is a major nosocomial pathogen that causes severe diarrheal disease. Though C. difficile is known to inhabit the human gastrointestinal tract, the mechanisms that allow this pathogen to adapt to the intestine and survive host defenses are not known. In this work, we investigated the response of C. difficile to the host defense peptide, LL-37, to determine the mechanisms underlying host adaptation and survival. Expression analyses revealed a previously unknown locus, which we named clnRAB, that is highly induced by LL-37 and acts as a global regulator of gene expression in C. difficile. Mutant analyses indicate that ClnRAB is a novel regulatory system that senses LL-37 as a host signal to regulate adaptation to the intestinal environment.
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| Overall design |
Strain 630∆erm without LL-37 served as the control strain. Expression in 630∆erm with LL-37 and expression in the clnR mutant without LL-37 were compared to this control strain. Expression in the clnR mutant with LL-37 was compared to 630∆erm with LL-37. Two independent replicates are included.
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| Contributor(s) |
Woods EC, Edwards AN, McBride SM, Billingsley JM, Patel N, Tharp GK |
| Citation missing |
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| Submission date |
Jun 11, 2018 |
| Last update date |
Jul 31, 2018 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
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| Phone |
404-727-7797
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| Organization name |
Yerkes National Primate Research Center
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| Department |
Developmental and Cognitive Neuroscience
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| Lab |
Genomics Core
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| Street address |
954 Gatewood Dr
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
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| Platforms (1) |
| GPL25175 |
Illumina HiSeq 3000 (Clostridioides difficile) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA475605 |
| SRA |
SRP150271 |
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